Information for Peptide: YSDEQLNSGR from APC_HUMAN

1. Biological Peptide

1.1 Native Sequence

    K.YSDEQLNSGR.Q

1.2 Peptide Properties

Start	Stop	MW	m/z for 2+	m/z for 3+	pI
1030	1039	1167.52	584.77	390.18	4.37

1.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
Y	b		y
S	b		y
D	b		y
E	b		y
Q	b		y
L	b		y
N	b		y
S	b		y
G	b		y
R	b		y

2. Synthetic Peptide

2.1 Synthetic (Internal Standard) Peptide Sequence

YSDEQLNSAR (Modification: G9 to A )

2.2 Peptide Properties

MW	m/z for 2+	m/z for 3+	pI
1181.53	591.77	394.85	4.37

2.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
Y	b		y
S	b		y
D	b		y
E	b		y
Q	b		y
L	b		y
N	b		y
S	b		y
A	b		y
R	b		y

2.4 Peptide Synthesis Report

Click to view/download synthesis report for YSDEQLNSGR

2.5 QqQ MS/MS

Transition Table:

Transition	m/z of Transition	Collision Energy
y9	1019.48	26.0
y8	932.44	22.0
y7	817.42	23.0
y6	688.37	24.0
y5	560.32	24.0
y4	447.23	21.0
y3	333.19	22.0

2.6 Calibration Curve of Standard

Description: A dilution series was run from 50 fmol to 0.1 fmol. The detection limit was determined to be 0.1 fmol.

 

Experiment Description: Cells from colon caner line HCT 116 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer. Internal standard has been synthesised, but has not been analyzed with sample.

2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times

2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides