1. Basic Information
| Name: | APC_HUMAN |
| Accession#: | P25054 |
| Description: | Adenomatous polyposis coli protein |
| AA Number: | 2842 |
| Sequence: |
1
51
101
151
201
251
301
351
401
451
501
551
601
651
701
751
801
851
901
951
1001
1051
1101
1151
1201
1251
1301
1351
1401
1451
1501
1551
1601
1651
1701
1751
1801
1851
1901
1951
2001
2051
2101
2151
2201
2251
2301
2351
2401
2451
2501
2551
2601
2651
2701
2751
2801
|
|
MAAASYDQLL KQVEALKMEN SNLRQELEDN SNHLTKLETE ASNMKEVLKQ
LQGSIEDEAM ASSGQIDLLE RLKELNLDSS NFPGVKLRSK MSLRSYGSRE
GSVSSRSGEC SPVPMGSFPR RGFVNGSRES TGYLEELEKE RSLLLADLDK
EEKEKDWYYA QLQNLTKRID SLPLTENFSL QTDMTRRQLE YEARQIRVAM
EEQLGTCQDM EKRAQRRIAR IQQIEKDILR IRQLLQSQAT EAERSSQNKH
ETGSHDAERQ NEGQGVGEIN MATSGNGQGS TTRMDHETAS VLSSSSTHSA
PRRLTSHLGT KVEMVYSLLS MLGTHDKDDM SRTLLAMSSS QDSCISMRQS
GCLPLLIQLL HGNDKDSVLL GNSRGSKEAR ARASAALHNI IHSQPDDKRG
RREIRVLHLL EQIRAYCETC WEWQEAHEPG MDQDKNPMPA PVEHQICPAV
CVLMKLSFDE EHRHAMNELG GLQAIAELLQ VDCEMYGLTN DHYSITLRRY
AGMALTNLTF GDVANKATLC SMKGCMRALV AQLKSESEDL QQVIASVLRN
LSWRADVNSK KTLREVGSVK ALMECALEVK KESTLKSVLS ALWNLSAHCT
ENKADICAVD GALAFLVGTL TYRSQTNTLA IIESGGGILR NVSSLIATNE
DHRQILRENN CLQTLLQHLK SHSLTIVSNA CGTLWNLSAR NPKDQEALWD
MGAVSMLKNL IHSKHKMIAM GSAAALRNLM ANRPAKYKDA NIMSPGSSLP
SLHVRKQKAL EAELDAQHLS ETFDNIDNLS PKASHRSKQR HKQSLYGDYV
FDTNRHDDNR SDNFNTGNMT VLSPYLNTTV LPSSSSSRGS LDSSRSEKDR
SLERERGIGL GNYHPATENP GTSSKRGLQI STTAAQIAKV MEEVSAIHTS
QEDRSSGSTT ELHCVTDERN ALRRSSAAHT HSNTYNFTKS ENSNRTCSMP
YAKLEYKRSS NDSLNSVSSS DGYGKRGQMK PSIESYSEDD ESKFCSYGQY
PADLAHKIHS ANHMDDNDGE LDTPNYSLKY SDEQLNSGRQ SPSQNERWAR
PKHIIEDEIK QSEQRQSRNQ STTYPVYTES TDDKHLKFQP HFGQQECVSP
YRSRGANGSE TNRVGSNHGI NQNVSQSLCQ EDDYEDDKPT NYSERYSEEE
QHEEEERPTN YSIKYNEEKR HVDQPIDYSL KYATDIPSSQ KQSFSFSKSS
SGQSSKTEHM SSSSENTSTP SSNAKRQNQL HPSSAQSRSG QPQKAATCKV
SSINQETIQT YCVEDTPICF SRCSSLSSLS SAEDEIGCNQ TTQEADSANT
LQIAEIKEKI GTRSAEDPVS EVPAVSQHPR TKSSRLQGSS LSSESARHKA
VEFSSGAKSP SKSGAQTPKS PPEHYVQETP LMFSRCTSVS SLDSFESRSI
ASSVQSEPCS GMVSGIISPS DLPDSPGQTM PPSRSKTPPP PPQTAQTKRE
VPKNKAPTAE KRESGPKQAA VNAAVQRVQV LPDADTLLHF ATESTPDGFS
CSSSLSALSL DEPFIQKDVE LRIMPPVQEN DNGNETESEQ PKESNENQEK
EAEKTIDSEK DLLDDSDDDD IEILEECIIS AMPTKSSRKA KKPAQTASKL
PPPVARKPSQ LPVYKLLPSQ NRLQPQKHVS FTPGDDMPRV YCVEGTPINF
STATSLSDLT IESPPNELAA GEGVRGGAQS GEFEKRDTIP TEGRSTDEAQ
GGKTSSVTIP ELDDNKAEEG DILAECINSA MPKGKSHKPF RVKKIMDQVQ
QASASSSAPN KNQLDGKKKK PTSPVKPIPQ NTEYRTRVRK NADSKNNLNA
ERVFSDNKDS KKQNLKNNSK VFNDKLPNNE DRVRGSFAFD SPHHYTPIEG
TPYCFSRNDS LSSLDFDDDD VDLSREKAEL RKAKENKESE AKVTSHTELT
SNQQSANKTQ AIAKQPINRG QPKPILQKQS TFPQSSKDIP DRGAATDEKL
QNFAIENTPV CFSHNSSLSS LSDIDQENNN KENEPIKETE PPDSQGEPSK
PQASGYAPKS FHVEDTPVCF SRNSSLSSLS IDSEDDLLQE CISSAMPKKK
KPSRLKGDNE KHSPRNMGGI LGEDLTLDLK DIQRPDSEHG LSPDSENFDW
KAIQEGANSI VSSLHQAAAA ACLSRQASSD SDSILSLKSG ISLGSPFHLT
PDQEEKPFTS NKGPRILKPG EKSTLETKKI ESESKGIKGG KKVYKSLITG
KVRSNSEISG QMKQPLQANM PSISRGRTMI HIPGVRNSSS STSPVSKKGP
PLKTPASKSP SEGQTATTSP RGAKPSVKSE LSPVARQTSQ IGGSSKAPSR
SGSRDSTPSR PAQQPLSRPI QSPGRNSISP GRNGISPPNK LSQLPRTSSP
STASTKSSGS GKMSYTSPGR QMSQQNLTKQ TGLSKNASSI PRSESASKGL
NQMNNGNGAN KKVELSRMSS TKSSGSESDR SERPVLVRQS TFIKEAPSPT
LRRKLEESAS FESLSPSSRP ASPTRSQAQT PVLSPSLPDM SLSTHSSVQA
GGWRKLPPNL SPTIEYNDGR PAKRHDIARS HSESPSRLPI NRSGTWKREH
SKHSSSLPRV STWRRTGSSS SILSASSESS EKAKSEDEKH VNSISGTKQS
KENQVSAKGT WRKIKENEFS PTNSTSQTVS SGATNGAESK TLIYQMAPAV
SKTEDVWVRI EDCPINNPRS GRSPTGNTPP VIDSVSEKAN PNIKDSKDNQ
AKQNVGNGSV PMRTVGLENR LNSFIQVDAP DQKGTEIKPG QNNPVPVSET
NESSIVERTP FSSSSSSKHS SPSGTVAARV TPFNYNPSPR KSSADSTSAR
PSQIPTPVNN NTKKRDSKTD STESSGTQSP KRHSGSYLVT SV |
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*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.
2. Protein Separation
Sample Preparation: Cells from colon caner line HCT 116 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.
3. LC-MS/MS Data
YSDEQLNSGR
No LC-MS/MS for this peptide.
HVDQPIDYSLK
No LC-MS/MS for this peptide.
QNQLHPSSAQSR
No LC-MS/MS for this peptide.
YATDIPSSQK
No LC-MS/MS for this peptide.
TPPPPPQTAQTK
No LC-MS/MS for this peptide.
ASAALHNIIHSQPDDK
No LC-MS/MS for this peptide.
QELEDNSNHLTK
No LC-MS/MS for this peptide.
HETGSHDAER
No LC-MS/MS for this peptide.
ESTGYLEELEK
No LC-MS/MS for this peptide.
SLLLADLDK
No LC-MS/MS for this peptide.
4. LC-MRM Screening
Peptides screening: Peptides and transitions for analysis were selected using a predictive peptide program. The results were screened for those with the highest specificity and the most intense transitions.
