1. Basic Information

Name:	UROM_HUMAN
Accession#:	P15311
Description:	Uromodulin
AA Number:	636
Sequence:	1 51 101 151 201 251 301 351 401 451 501 551 601    MGQPSLTWML MVVVASWFIT TAATDTSEAR WCSECHSNAT CTEDEAVTTC TCQEGFTGDG TCVDLDECAI PGAHNCSANS SCVNTPGSFS CVCPEGFRLS PGLGCTDVDE CAEPGLSHCA LATCVNVVGS YLCVCPAGYR GDGWHCECSP GSCGPGLDCV PEGDALVCAD PCQAHRTLEY WRSTEYGEGY ACDTDLRGWY RFVGQGGARM AETCVPVLRC NTAAPMWLNG THPSSDEGIV SRKACAHWSG HCCLWDASVQ VKACAGGYYV YNLTAPPECH LAYCTDPSSV EGTCEECSID EDCKSNNGRW HCQCKQDFNI TDISLLEHRL ECGANDMKVS LGKCQLKSLG FDKVFMYLSD SRCSGFNDRD NRDWVSVVTP ARDGPCGTVL TRNETHATYS NTLYLADEII IRDLNIKINF ACSYPLDMKV SLKTALQPMV SALNIRVGGT GMFTVRMALF QTPSYTQPYQ GSSVTLSTEA FLYVGTMLDG GDLSRFALLM TNCYATPSSN ATDPLKYFII QDRCPHTRDS TIQVVENESS QGRFSVQMFR FAGNYDLVYL HCEVYLCDTM NEKCKPTCSG TRFRSGSVID QSRVLNLGPI TRKGVQATVS RAFSSLGLLK VWLPLLLSAT LTLTFQ

*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.

2. Protein Separation

Sample Preparation: Control urine was processed via ultrafiltration according to Moffitt Proteomics protocol and subjected to in-solution digestion with trypsin, following thermal denaturation, reduction, and alkylation.

 

3. LC-MS/MS Data

DWVSVVTPAR

 

VLNLGPITR

 

4. LC-MRM Screening

Peptides screening: Peptides were chosen in Skyline with preference given to tryptic sequences observed in the NIST Human LC-MS/MS library; for such peptides, the 5 most abundant y ions were automatically chosen. Additional peptides were chosen with preference given to sequences between 8 - 15 amino acids long, with no missed cleavages, and with no methionines. Fragment ions were chosen to represent the largest y ions, with attention to the presence of P, D, E, and H residues in the sequence.