1. Basic Information

Name:	TF65_HUMAN
Accession#:	Q04206
Description:	Transcription factor p65
AA Number:	551
Sequence:	1 51 101 151 201 251 301 351 401 451 501    MDELFPLIFP AEPAQASGPY VEIIEQPKQR GMRFRYKCEG RSAGSIPGER STDTTKTHPT IKINGYTGPG TVRISLVTKD PPHRPHPHEL VGKDCRDGFY EAELCPDRCI HSFQNLGIQC VKKRDLEQAI SQRIQTNNNP FQVPIEEQRG DYDLNAVRLC FQVTVRDPSG RPLRLPPVLS HPIFDNRAPN TAELKICRVN RNSGSCLGGD EIFLLCDKVQ KEDIEVYFTG PGWEARGSFS QADVHRQVAI VFRTPPYADP SLQAPVRVSM QLRRPSDREL SEPMEFQYLP DTDDRHRIEE KRKRTYETFK SIMKKSPFSG PTDPRPPPRR IAVPSRSSAS VPKPAPQPYP FTSSLSTINY DEFPTMVFPS GQISQASALA PAPPQVLPQA PAPAPAPAMV SALAQAPAPV PVLAPGPPQA VAPPAPKPTQ AGEGTLSEAL LQLQFDDEDL GALLGNSTDP AVFTDLASVD NSEFQQLLNQ GIPVAPHTTE PMLMEYPEAI TRLVTGAQRP PDPAPAPLGA PGLPNGLLSG DEDFSSIADM DFSALLSQIS S

*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.

2. Protein Separation

Sample Preparation: Cells from line 8226 were lysed in RIPA buffer. The equivalent of 50,000 cells were loaded onto an SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.

 

3. LC-MS/MS Data

QVAIVFR

    No LC-MS/MS for this peptide.

INGYTGPGTVR

    No LC-MS/MS for this peptide.

GDYDLNAVR

    No LC-MS/MS for this peptide.

IQTNNNPFQVPIEEQR

    No LC-MS/MS for this peptide.

APNTAELK

    No LC-MS/MS for this peptide.

TPPYADPSLQAPVR

    No LC-MS/MS for this peptide.

LPPVLSHPIFDNR

    No LC-MS/MS for this peptide.

DLEQAISQR

    No LC-MS/MS for this peptide.

GSFSQADVHR

    No LC-MS/MS for this peptide.

4. LC-MRM Screening

Peptides screening: Peptides and transitions for analysis were selected using both LC-MS/MS data and a predictive peptide program. The results were screened for those with the highest specificity and the most intense transitions.