1. Basic Information

Name:	TCF25_HUMAN
Accession#:	Q9BQ70
Description:	Transcription factor 25
AA Number:	676
Sequence:	1 51 101 151 201 251 301 351 401 451 501 551 601 651    MSRRALRRLR GEQRGQEPLG PGALHFDLRD DDDAEEEGPK RELGVRRPGG AGKEGVRVNN RFELINIDDL EDDPVVNGER SGCALTDAVA PGNKGRGQRG NTESKTDGDD TETVPSEQSH ASGKLRKKKK KQKNKKSSTG EASENGLEDI DRILERIEDS TGLNRPGPAP LSSRKHVLYV EHRHLNPDTE LKRYFGARAI LGEQRPRQRQ RVYPKCTWLT TPKSTWPRYS KPGLSMRLLE SKKGLSFFAF EHSEEYQQAQ HKFLVAVESM EPNNIVVLLQ TSPYHVDSLL QLSDACRFQE DQEMARDLVE RALYSMECAF HPLFSLTSGA CRLDYRRPEN RSFYLALYKQ MSFLEKRGCP RTALEYCKLI LSLEPDEDPL CMLLLIDHLA LRARNYEYLI RLFQEWEAHR NLSQLPNFAF SVPLAYFLLS QQTDLPECEQ SSARQKASLL IQQALTMFPG VLLPLLESCS VRPDASVSSH RFFGPNAEIS QPPALSQLVN LYLGRSHFLW KEPATMSWLE ENVHEVLQAV DAGDPAVEAC ENRRKVLYQR APRNIHRHVI LSEIKEAVAA LPPDVTTQSV MGFDPLPPSD TIYSYVRPER LSPISHGNTI ALFFRSLLPN YTMEGERPEE GVAGGLNRNQ GLNRLMLAVR DMMANFHLND LEAPHEDDAE GEGEWD

*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.

2. Protein Separation

Sample Preparation: Cells from HT116 colon cancer cells were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/6 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.

 

3. LC-MS/MS Data

NYEYLIR

    No LC-MS/MS for this peptide.

AILGEQRPR

    No LC-MS/MS for this peptide.

HVILSEIK

    No LC-MS/MS for this peptide.

HLNPDTELK

    No LC-MS/MS for this peptide.

4. LC-MRM Screening

Peptides screening: Peptides and transitions for analysis were selected using a predictive peptide program. The results were screened for those with the highest specificity and the most intense transitions.