1. Basic Information

Name:	PUMA_HUMAN
Accession#:	Q9BXH1
Description:	Bcl-2-binding component 3
AA Number:	131
Sequence:	1 51 101    MKFGMGSAQA CPCQVPRAAS TTWVPCQICG PQPSLSLAEQ HLESPVPSAP GALAGGPTQA APGVRGEEEQ WAREIGAQLR RMADDLNAQY ERRRQEEQQR HRPSPWRVLY NLIMGLLPLP RGHRAPEMEP N

*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.

2. Protein Separation

Sample Preparation: Cells from 8226 cells were lysed in RIPA buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.

 

3. LC-MS/MS Data

VLYNLIMGLLPLPR

    No LC-MS/MS for this peptide.

4. LC-MRM Screening

Peptides screening: Peptides and transitions for analysis were selected using a predictive peptide program. The results were screened for those with the highest specificity and the most intense transitions.