1. Basic Information
| Name: | NCOR2_HUMAN |
| Accession#: | Q9Y618 |
| Description: | Nuclear receptor corepressor 2 |
| AA Number: | 2525 |
| Sequence: |
1
51
101
151
201
251
301
351
401
451
501
551
601
651
701
751
801
851
901
951
1001
1051
1101
1151
1201
1251
1301
1351
1401
1451
1501
1551
1601
1651
1701
1751
1801
1851
1901
1951
2001
2051
2101
2151
2201
2251
2301
2351
2401
2451
2501
|
|
MSGSTQPVAQ TWRATEPRYP PHSLSYPVQI ARTHTDVGLL EYQHHSRDYA
SHLSPGSIIQ PQRRRPSLLS EFQPGNERSQ ELHLRPESHS YLPELGKSEM
EFIESKRPRL ELLPDPLLRP SPLLATGQPA GSEDLTKDRS LTGKLEPVSP
PSPPHTDPEL ELVPPRLSKE ELIQNMDRVD REITMVEQQI SKLKKKQQQL
EEEAAKPPEP EKPVSPPPIE SKHRSLVQII YDENRKKAEA AHRILEGLGP
QVELPLYNQP SDTRQYHENI KINQAMRKKL ILYFKRRNHA RKQWEQKFCQ
RYDQLMEAWE KKVERIENNP RRRAKESKVR EYYEKQFPEI RKQRELQERM
QSRVGQRGSG LSMSAARSEH EVSEIIDGLS EQENLEKQMR QLAVIPPMLY
DADQQRIKFI NMNGLMADPM KVYKDRQVMN MWSEQEKETF REKFMQHPKN
FGLIASFLER KTVAECVLYY YLTKKNENYK SLVRRSYRRR GKSQQQQQQQ
QQQQQQQQQQ PMPRSSQEEK DEKEKEKEAE KEEEKPEVEN DKEDLLKEKT
DDTSGEDNDE KEAVASKGRK TANSQGRRKG RITRSMANEA NSEEAITPQQ
SAELASMELN ESSRWTEEEM ETAKKGLLEH GRNWSAIARM VGSKTVSQCK
NFYFNYKKRQ NLDEILQQHK LKMEKERNAR RKKKKAPAAA SEEAAFPPVV
EDEEMEASGV SGNEEEMVEE AEALHASGNE VPRGECSGPA TVNNSSDTES
IPSPHTEAAK DTGQNGPKPP ATLGADGPPP GPPTPPPEDI PAPTEPTPAS
EATGAPTPPP APPSPSAPPP VVPKEEKEEE TAAAPPVEEG EEQKPPAAEE
LAVDTGKAEE PVKSECTEEA EEGPAKGKDA EAAEATAEGA LKAEKKEGGS
GRATTAKSSG APQDSDSSAT CSADEVDEAE GGDKNRLLSP RPSLLTPTGD
PRANASPQKP LDLKQLKQRA AAIPPIQVTK VHEPPREDAA PTKPAPPAPP
PPQNLQPESD APQQPGSSPR GKSRSPAPPA DKEAEKPVFF PAFAAEAQKL
PGDPPCWTSG LPFPVPPREV IKASPHAPDP SAFSYAPPGH PLPLGLHDTA
RPVLPRPPTI SNPPPLISSA KHPSVLERQI GAISQGMSVQ LHVPYSEHAK
APVGPVTMGL PLPMDPKKLA PFSGVKQEQL SPRGQAGPPE SLGVPTAQEA
SVLRGTALGS VPGGSITKGI PSTRVPSDSA ITYRGSITHG TPADVLYKGT
ITRIIGEDSP SRLDRGREDS LPKGHVIYEG KKGHVLSYEG GMSVTQCSKE
DGRSSSGPPH ETAAPKRTYD MMEGRVGRAI SSASIEGLMG RAIPPERHSP
HHLKEQHHIR GSITQGIPRS YVEAQEDYLR REAKLLKREG TPPPPPPSRD
LTEAYKTQAL GPLKLKPAHE GLVATVKEAG RSIHEIPREE LRHTPELPLA
PRPLKEGSIT QGTPLKYDTG ASTTGSKKHD VRSLIGSPGR TFPPVHPLDV
MADARALERA CYEESLKSRP GTASSSGGSI ARGAPVIVPE LGKPRQSPLT
YEDHGAPFAG HLPRGSPVTT REPTPRLQEG SLSSSKASQD RKLTSTPREI
AKSPHSTVPE HHPHPISPYE HLLRGVSGVD LYRSHIPLAF DPTSIPRGIP
LDAAAAYYLP RHLAPNPTYP HLYPPYLIRG YPDTAALENR QTIINDYITS
QQMHHNAATA MAQRADMLRG LSPRESSLAL NYAAGPRGII DLSQVPHLPV
LVPPTPGTPA TAMDRLAYLP TAPQPFSSRH SSSPLSPGGP THLTKPTTTS
SSERERDRDR ERDRDREREK SILTSTTTVE HAPIWRPGTE QSSGSSGSSG
GGGGSSSRPA SHSHAHQHSP ISPRTQDALQ QRPSVLHNTG MKGIITAVEP
STPTVLRSTS TSSPVRPAAT FPPATHCPLG GTLDGVYPTL MEPVLLPKEA
PRVARPERPR ADTGHAFLAK PPARSGLEPA SSPSKGSEPR PLVPPVSGHA
TIARTPAKNL APHHASPDPP APPASASDPH REKTQSKPFS IQELELRSLG
YHGSSYSPEG VEPVSPVSSP SLTHDKGLPK HLEELDKSHL EGELRPKQPG
PVKLGGEAAH LPHLRPLPES QPSSSPLLQT APGVKGHQRV VTLAQHISEV
ITQDYTRHHP QQLSAPLPAP LYSFPGASCP VLDLRRPPSD LYLPPPDHGA
PARGSPHSEG GKRSPEPNKT SVLGGGEDGI EPVSPPEGMT EPGHSRSAVY
PLLYRDGEQT EPSRMGSKSP GNTSQPPAFF SKLTESNSAM VKSKKQEINK
KLNTHNRNEP EYNISQPGTE IFNMPAITGT GLMTYRSQAV QEHASTNMGL
EAIIRKALMG KYDQWEESPP LSANAFNPLN ASASLPAAMP ITAADGRSDH
TLTSPGGGGK AKVSGRPSSR KAKSPAPGLA SGDRPPSVSS VHSEGDCNRR
TPLTNRVWED RPSSAGSTPF PYNPLIMRLQ AGVMASPPPP GLPAGSGPLA
GPHHAWDEEP KPLLCSQYET LSDSE |
|
*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.
2. Protein Separation
Sample Preparation: Cells from colon caner line HCT 116 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 60,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/6 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.
3. LC-MS/MS Data
GTALGSVPGGSITK
No LC-MS/MS for this peptide.
GSITHGTPADVLYK
No LC-MS/MS for this peptide.
4. LC-MRM Screening
Peptides screening: Peptides and transitions for analysis were selected using a predictive peptide program. The results were screened for those with the highest specificity and the most intense transitions.
