1. Basic Information
| Name: | MP2K1_HUMAN | |||
| Accession#: | Q02750 | |||
| Description: | Dual specificity mitogen-activated protein kinase kinase 1 | |||
| AA Number: | 393 | |||
| Sequence: |
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*Green background amino acids are PTMs.
2. Protein Separation
Sample Preparation: Cells from Melanoma cell lines were lysed in RIPA buffer. The equivalent of 200,000 cells were loaded onto an SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/6 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.
3. LC-MS/MS Data
LEAFLTQK
No LC-MS/MS for this peptide.
DVKPSNILVNSR
No LC-MS/MS for this peptide.
LEELELDEQQR
No LC-MS/MS for this peptide.
YPIPPPDAK
No LC-MS/MS for this peptide.
VSHKPSGLVMAR
No LC-MS/MS for this peptide.
IPEQILGK
No LC-MS/MS for this peptide.
LIHLEIKPAIR
No LC-MS/MS for this peptide.
VSIAVIK
No LC-MS/MS for this peptide.
4. LC-MRM Screening
Peptides screening: Peptides and transitions for analysis were selected using a predictive peptide program. The results were screened for those with the highest specificity and the most intense transitions.
