1. Basic Information
| Name: | MK01_HUMAN | |||
| Accession#: | P28482 | |||
| Description: | Mitogen-activated protein kinase 1 | |||
| AA Number: | 360 | |||
| Sequence: |
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*Green background amino acids are PTMs.
2. Protein Separation
Sample Preparation: Cells from Melanoma cell lines were lysed in 8M urea/100mM Ammonium bicarbonate buffer. The equivalent of 200,000 cells were loaded onto an SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/6 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.
3. LC-MS/MS Data
NYLLSLPHK
No LC-MS/MS for this peptide.
LFPNADSK
No LC-MS/MS for this peptide.
ALDLLDK
No LC-MS/MS for this peptide.
HENIIGINDIIR
No LC-MS/MS for this peptide.
VADPDHDHTGFLTEYVATR
No LC-MS/MS for this peptide.
APEIMLNSK
No LC-MS/MS for this peptide.
ELIFEETAR
No LC-MS/MS for this peptide.
APTIEQMK
No LC-MS/MS for this peptide.
GQVFDVGPR
No LC-MS/MS for this peptide.
YIHSANVLHR
No LC-MS/MS for this peptide.
4. LC-MRM Screening
Peptides screening: Peptides and transitions for analysis were selected using a predictive peptide program. The results were screened for those with the highest specificity and the most intense transitions.
