1. Basic Information
| Name: | MINT_HUMAN |
| Accession#: | Q96T58 |
| Description: | Msx2-interacting protein |
| AA Number: | 3664 |
| Sequence: |
1
51
101
151
201
251
301
351
401
451
501
551
601
651
701
751
801
851
901
951
1001
1051
1101
1151
1201
1251
1301
1351
1401
1451
1501
1551
1601
1651
1701
1751
1801
1851
1901
1951
2001
2051
2101
2151
2201
2251
2301
2351
2401
2451
2501
2551
2601
2651
2701
2751
2801
2851
2901
2951
3001
3051
3101
3151
3201
3251
3301
3351
3401
3451
3501
3551
3601
3651
|
|
MVRETRHLWV GNLPENVREE KIIEHFKRYG RVESVKILPK RGSEGGVAAF
VDFVDIKSAQ KAHNSVNKMG DRDLRTDYNE PGTIPSAARG LDDTVSIASR
SREVSGFRGG GGGPAYGPPP SLHAREGRYE RRLDGASDNR ERAYEHSAYG
HHERGTGGFD RTRHYDQDYY RDPRERTLQH GLYYASRSRS PNRFDAHDPR
YEPRAREQFT LPSVVHRDIY RDDITREVRG RRPERNYQHS RSRSPHSSQS
RNQSPQRLAS QASRPTRSPS GSGSRSRSSS SDSISSSSST SSDSSDSSSS
SSDDSPARSV QSAAVPAPTS QLLSSLEKDE PRKSFGIKVQ NLPVRSTDTS
LKDGLFHEFK KFGKVTSVQI HGTSEERYGL VFFRQQEDQE KALTASKGKL
FFGMQIEVTA WIGPETESEN EFRPLDERID EFHPKATRTL FIGNLEKTTT
YHDLRNIFQR FGEIVDIDIK KVNGVPQYAF LQYCDIASVC KAIKKMDGEY
LGNNRLKLGF GKSMPTNCVW LDGLSSNVSD QYLTRHFCRY GPVVKVVFDR
LKGMALVLYN EIEYAQAAVK ETKGRKIGGN KIKVDFANRE SQLAFYHCME
KSGQDIRDFY EMLAERREER RASYDYNQDR TYYESVRTPG TYPEDSRRDY
PARGREFYSE WETYQGDYYE SRYYDDPREY RDYRNDPYEQ DIREYSYRQR
ERERERERFE SDRDRDHERR PIERSQSPVH LRRPQSPGAS PSQAERLPSD
SERRLYSRSS DRSGSCSSLS PPRYEKLDKS RLERYTKNEK TDKERTFDPE
RVERERRLIR KEKVEKDKTD KQKRKGKVHS PSSQSSETDQ ENEREQSPEK
PRSCNKLSRE KADKEGIAKN RLELMPCVVL TRVKEKEGKV IDHTPVEKLK
AKLDNDTVKS SALDQKLQVS QTEPAKSDLS KLESVRMKVP KEKGLSSHVE
VVEKEGRLKA RKHLKPEQPA DGVSAVDLEK LEARKRRFAD SNLKAEKQKP
EVKKSSPEME DARVLSKKQP DVSSREVILL REGEAERKPV RKEILKRESK
KIKLDRLNTV ASPKDCQELA SISVGSGSRP SSDLQARLGE LAGESVENQE
VQSKKPIPSK PQLKQLQVLD DQGPEREDVR KNYCSLRDET PERKSGQEKS
HSVNTEEKIG IDIDHTQSYR KQMEQSRRKQ QMEMEIAKSE KFGSPKKDVD
EYERRSLVHE VGKPPQDVTD DSPPSKKKRM DHVDFDICTK RERNYRSSRQ
ISEDSERTGG SPSVRHGSFH EDEDPIGSPR LLSVKGSPKV DEKVLPYSNI
TVREESLKFN PYDSSRREQM ADMAKIKLSV LNSEDELNRW DSQMKQDAGR
FDVSFPNSII KRDSLRKRSV RDLEPGEVPS DSDEDGEHKS HSPRASALYE
SSRLSFLLRD REDKLRERDE RLSSSLERNK FYSFALDKTI TPDTKALLER
AKSLSSSREE NWSFLDWDSR FANFRNNKDK EKVDSAPRPI PSWYMKKKKI
RTDSEGKMDD KKEDHKEEEQ ERQELFASRF LHSSIFEQDS KRLQHLERKE
EDSDFISGRI YGKQTSEGAN STTDSIQEPV VLFHSRFMEL TRMQQKEKEK
DQKPKEVEKQ EDTENHPKTP ESAPENKDSE LKTPPSVGPP SVTVVTLESA
PSALEKTTGD KTVEAPLVTE EKTVEPATVS EEAKPASEPA PAPVEQLEQV
DLPPGADPDK EAAMMPAGVE EGSSGDQPPY LDAKPPTPGA SFSQAESNVD
PEPDSTQPLS KPAQKSEEAN EPKAEKPDAT ADAEPDANQK AEAAPESQPP
ASEDLEVDPP VAAKDKKPNK SKRSKTPVQA AAVSIVEKPV TRKSERIDRE
KLKRSNSPRG EAQKLLELKM EAEKITRTAS KNSAADLEHP EPSLPLSRTR
RRNVRSVYAT MGDHENRSPV KEPVEQPRVT RKRLERELQE AAAVPTTPRR
GRPPKTRRRA DEEEENEAKE PAETLKPPEG WRSPRSQKTA AGGGPQGKKG
KNEPKVDATR PEATTEVGPQ IGVKESSMEP KAAEEEAGSE QKRDRKDAGT
DKNPPETAPV EVVEKKPAPE KNSKSKRGRS RNSRLAVDKS ASLKNVDAAV
SPRGAAAQAG ERESGVVAVS PEKSESPQKE DGLSSQLKSD PVDPDKEPEK
EDVSASGPSP EATQLAKQME LEQAVEHIAK LAEASASAAY KADAPEGLAP
EDRDKPAHQA SETELAAAIG SIINDISGEP ENFPAPPPYP GESQTDLQPP
AGAQALQPSE EGMETDEAVS GILETEAATE SSRPPVNAPD PSAGPTDTKE
ARGNSSETSH SVPEAKGSKE VEVTLVRKDK GRQKTTRSRR KRNTNKKVVA
PVESHVPESN QAQGESPAAN EGTTVQHPEA PQEEKQSEKP HSTPPQSCTS
DLSKIPSTEN SSQEISVEER TPTKASVPPD LPPPPQPAPV DEEPQARFRV
HSIIESDPVT PPSDPSIPIP TLPSVTAAKL SPPVASGGIP HQSPPTKVTE
WITRQEEPRA QSTPSPALPP DTKASDVDTS SSTLRKILMD PKYVSATSVT
STSVTTAIAE PVSAAPCLHE APPPPVDSKK PLEEKTAPPV TNNSEIQASE
VLVAADKEKV APVIAPKITS VISRMPVSID LENSQKITLA KPAPQTLTGL
VSALTGLVNV SLVPVNALKG PVKGSVTTLK SLVSTPAGPV NVLKGPVNVL
TGPVNVLTTP VNATVGTVNA APGTVNAAAS AVNATASAVT VTAGAVTAAS
GGVTATTGTV TMAGAVIAPS TKCKQRASAN ENSRFHPGSM PVIDDRPADA
GSGAGLRVNT SEGVVLLSYS GQKTEGPQRI SAKISQIPPA SAMDIEFQQS
VSKSQVKPDS VTASQPPSKG PQAPAGYANV ATHSTLVLTA QTYNASPVIS
SVKADRPSLE KPEPIHLSVS TPVTQGGTVK VLTQGINTPP VLVHNQLVLT
PSIVTTNKKL ADPVTLKIET KVLQPANLGS TLTPHHPPAL PSKLPTEVNH
VPSGPSIPAD RTVSHLAAAK LDAHSPRPSG PGPSSFPRAS HPSSTASTAL
STNATVMLAA GIPVPQFISS IHPEQSVIMP PHSITQTVSL SHLSQGEVRM
NTPTLPSITY SIRPEALHSP RAPLQPQQIE VRAPQRASTP QPAPAGVPAL
ASQHPPEEEV HYHLPVARAT APVQSEVLVM QSEYRLHPYT VPRDVRIMVH
PHVTAVSEQP RAADGVVKVP PASKAPQQPG KEAAKTPDAK AAPTPTPAPV
PVPVPLPAPA PAPHGEARIL TVTPSNQLQG LPLTPPVVVT HGVQIVHSSG
ELFQEYRYGD IRTYHPPAQL THTQFPAASS VGLPSRTKTA AQGPPPEGEP
LQPPQPVQST QPAQPAPPCP PSQLGQPGQP PSSKMPQVSQ EAKGTQTGVE
QPRLPAGPAN RPPEPHTQVQ RAQAETGPTS FPSPVSVSMK PDLPVSLPTQ
TAPKQPLFVP TTSGPSTPPG LVLPHTEFQP APKQDSSPHL TSQRPVDMVQ
LLKKYPIVWQ GLLALKNDTA AVQLHFVSGN NVLAHRSLPL SEGGPPLRIA
QRMRLEATQL EGVARRMTVE TDYCLLLALP CGRDQEDVVS QTESLKAAFI
TYLQAKQAAG IINVPNPGSN QPAYVLQIFP PCEFSESHLS RLAPDLLASI
SNISPHLMIV IASV |
|
*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.
2. Protein Separation
Sample Preparation: Cells from colon caner line HCT 116 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 60,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/6 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.
3. LC-MS/MS Data
TPGTYPEDSR
No LC-MS/MS for this peptide.
SQSPVHLR
No LC-MS/MS for this peptide.
TDYNEPGTIPSAAR
No LC-MS/MS for this peptide.
GGGGGPAYGPPPSLHAR
No LC-MS/MS for this peptide.
LPSDSER
No LC-MS/MS for this peptide.
4. LC-MRM Screening
Peptides screening: Peptides and transitions for analysis were selected using a predictive peptide program. The results were screened for those with the highest specificity and the most intense transitions.
