1. Basic Information
| Name: | MAML3_HUMAN |
| Accession#: | Q96JK9 |
| Description: | Mastermind-like protein 3 |
| AA Number: | 1134 |
| Sequence: |
1
51
101
151
201
251
301
351
401
451
501
551
601
651
701
751
801
851
901
951
1001
1051
1101
|
|
MGDFAAPAAA ANGSSICINS SLNSSLGGAG IGVNNTPNST PAAPSSNHPA
AGGCGGSGGP GGGSAAVPKH STVVERLRQR IEGCRRHHVN CENRYQQAQV
EQLELERRDT VSLYQRTLEQ RAKKSGAGTG KQQHPSKPQQ DAEAASAEQR
NHTLIMLQET VKRKLEGARS PLNGDQQNGA CDGNFSPTSK RIRKDISAGM
EAINNLPSNM PLPSASPLHQ LDLKPSLPLQ NSGTHTPGLL EDLSKNGRLP
EIKLPVNGCS DLEDSFTILQ SKDLKQEPLD DPTCIDTSET SLSNQNKLFS
DINLNDQEWQ ELIDELANTV PEDDIQDLFN EDFEEKKEPE FSQPATETPL
SQESASVKSD PSHSPFAHVS MGSPQARPSS SGPPFSTVST ATSLPSVAST
PAAPNPASSP ANCAVQSPQT PNQAHTPGQA PPRPGNGYLL NPAAVTVAGS
ASGPVAVPSS DMSPAEQLKQ MAAQQQQRAK LMQQKQQQQQ QQQQQQQQQQ
QQQQQQHSNQ TSNWSPLGPP SSPYGAAFTA EKPNSPMMYP QAFNNQNPIV
PPMANNLQKT TMNNYLPQNH MNMINQQPNN LGTNSLNKQH NILTYGNTKP
LTHFNADLSQ RMTPPVANPN KNPLMPYIQQ QQQQQQQQQQ QQQQQQPPPP
QLQAPRAHLS EDQKRLLLMK QKGVMNQPMA YAALPSHGQE QHPVGLPRTT
GPMQSSVPPG SGGMVSGASP AGPGFLGSQP QAAIMKQMLI DQRAQLIEQQ
KQQFLREQRQ QQQQQQQQIL AEQQLQQSHL PRQHLQPQRN PYPVQQVNQF
QGSPQDIAAV RSQAALQSMR TSRLMAQNAG MMGIGPSQNP GTMATAAAQS
EMGLAPYSTT PTSQPGMYNM STGMTQMLQH PNQSGMSITH NQAQGPRQPA
SGQGVGMVSG FGQSMLVNSA ITQQHPQMKG PVGQALPRPQ APPRLQSLMG
TVQQGAQSWQ QRSLQGMPGR TSGELGPFNN GASYPLQAGQ PRLTKQHFPQ
GLSQSVVDAN TGTVRTLNPA AMGRQMMPSL PGQQGTSQAR PMVMSGLSQG
VPGMPAFSQP PAQQQIPSGS FAPSSQSQAY ERNAPQDVSY NYSGDGAGGS
FPGLPDGADL VDSIIKGGPG DEWMQELDEL FGNP |
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*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.
2. Protein Separation
Sample Preparation: Cells from HT116 colon cancer cells were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 60,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/6 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.
3. LC-MS/MS Data
GPVGQALPRPQAPPR
No LC-MS/MS for this peptide.
4. LC-MRM Screening
Peptides screening: Peptides and transitions for analysis were selected using a predictive peptide program. The results were screened for those with the highest specificity and the most intense transitions.
