1. Basic Information
| Name: | MAML2_HUMAN |
| Accession#: | Q8IZL2 |
| Description: | Mastermind-like protein 2 |
| AA Number: | 1153 |
| Sequence: |
1
51
101
151
201
251
301
351
401
451
501
551
601
651
701
751
801
851
901
951
1001
1051
1101
1151
|
|
MGDTAPPQAP AGGLGGASGA GLLGGGSVTP RVHSAIVERL RARIAVCRQH
HLSCEGRYER GRAESSDRER ESTLQLLSLV QHGQGARKAG KHTKATATAA
TTTAPPPPPA APPAASQAAA TAAPPPPPDY HHHHQQHLLN SSNNGGSGGI
NGEQQPPAST PGDQRNSALI ALQGSLKRKQ VVNLSPANSK RPNGFVDNSF
LDIKRIRVGE NLSAGQGGLQ INNGQSQIMS GTLPMSQAPL RKTNTLPSHT
HSPGNGLFNM GLKEVKKEPG ETLSCSKHMD GQMTQENIFP NRYGDDPGEQ
LMDPELQELF NELTNISVPP MSDLELENMI NATIKQDDPF NIDLGQQSQR
STPRPSLPME KIVIKSEYSP GLTQGPSGSP QLRPPSAGPA FSMANSALST
SSPIPSVPQS QAQPQTGSGA SRALPSWQEV SHAQQLKQIA ANRQQHARMQ
QHQQQHQPTN WSALPSSAGP SPGPFGQEKI PSPSFGQQTF SPQSSPMPGV
AGGSGQSKVM ANYMYKAGPS AQGGHLDVLM QQKPQDLSRS FINNPHPAME
PRQGNTKPLF HFNSDQANQQ MPSVLPSQNK PSLLHYTQQQ QQQQQQQQQQ
QQQQQQQQQQ QQQQQQQQSS ISAQQQQQQQ SSISAQQQQQ QQQQQQQQQQ
QQQQQQQQQQ QQPSSQPAQS LPSQPLLRSP LPLQQKLLLQ QMQNQPIAGM
GYQVSQQQRQ DQHSVVGQNT GPSPSPNPCS NPNTGSGYMN SQQSLLNQQL
MGKKQTLQRQ IMEQKQQLLL QQQMLADAEK IAPQDQINRH LSRPPPDYKD
QRRNVGNMQP TAQYSGGSST ISLNSNQALA NPVSTHTILT PNSSLLSTSH
GTRMPSLSTA VQNMGMYGNL PCNQPNTYSV TSGMNQLTQQ RNPKQLLANQ
NNPMMPRPPT LGPSNNNNVA TFGAGSVGNS QQLRPNLTHS MASMPPQRTS
NVMITSNTTA PNWASQEGTS KQQEALTSAG VRFPTGTPAA YTPNQSLQQA
VGSQQFSQRA VAPPNQLTPA VQMRPMNQMS QTLNGQTMGP LRGLNLRPNQ
LSTQILPNLN QSGTGLNQSR TGINQPPSLT PSNFPSPNQS SRAFQGTDHS
SDLAFDFLSQ QNDNMGPALN SDADFIDSLL KTEPGNDDWM KDINLDEILG
NNS |
|
*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.
2. Protein Separation
Sample Preparation: Cells from HT116 colon cancer cells were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 60,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/6 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.
3. LC-MS/MS Data
QVVNLSPANSK
No LC-MS/MS for this peptide.
4. LC-MRM Screening
Peptides screening: Peptides and transitions for analysis were selected using a predictive peptide program. The results were screened for those with the highest specificity and the most intense transitions.
