1. Basic Information
| Name: | JAK2_HUMAN |
| Accession#: | O60674 |
| Description: | Tyrosine-protein kinase JAK2 |
| AA Number: | 1140 |
| Sequence: |
1
51
101
151
201
251
301
351
401
451
501
551
601
651
701
751
801
851
901
951
1001
1051
1101
|
|
SEQUENCEMG MACLTMTEME GTSTSSIYQN GDISGNANSM KQIDPVLQVY
LYHSLGKSEA DYLTFPSGEY VAEEICIAAS KACGITPVYH NMFALMSETE
RIWYPPNHVF HIDESTRHNV LYRIRFYFPR WYCSGSNRAY RHGISRGAEA
PLLDDFVMSY LFAQWRHDFV HGWIKVPVTH ETQEECLGMA VLDMMRIAKE
NDQTPLAIYN SISYKTFLPK CIRAKIQDYH ILTRKRIRYR FRRFIQQFSQ
CKATARNLKL KYLINLETLQ SAFYTEKFEV KEPGSGPSGE EIFATIIITG
NGGIQWSRGK HKESETLTEQ DLQLYCDFPN IIDVSIKQAN QEGSNESRVV
TIHKQDGKNL EIELSSLREA LSFVSLIDGY YRLTADAHHY LCKEVAPPAV
LENIQSNCHG PISMDFAISK LKKAGNQTGL YVLRCSPKDF NKYFLTFAVE
RENVIEYKHC LITKNENEEY NLSGTKKNFS SLKDLLNCYQ METVRSDNII
FQFTKCCPPK PKDKSNLLVF RTNGVSDVPT SPTLQRPTHM NQMVFHKIRN
EDLIFNESLG QGTFTKIFKG VRREVGDYGQ LHETEVLLKV LDKAHRNYSE
SFFEAASMMS KLSHKHLVLN YGVCVCGDEN ILVQEFVKFG SLDTYLKKNK
NCINILWKLE VAKQLAWAMH FLEENTLIHG NVCAKNILLI REEDRKTGNP
PFIKLSDPGI SITVLPKDIL QERIPWVPPE CIENPKNLNL ATDKWSFGTT
LWEICSGGDK PLSALDSQRK LQFYEDRHQL PAPKWAELAN LINNCMDYEP
DFRPSFRAII RDLNSLFTPD YELLTENDML PNMRIGALGF SGAFEDRDPT
QFEERHLKFL QQLGKGNFGS VEMCRYDPLQ DNTGEVVAVK KLQHSTEEHL
RDFEREIEIL KSLQHDNIVK YKGVCYSAGR RNLKLIMEYL PYGSLRDYLQ
KHKERIDHIK LLQYTSQICK GMEYLGTKRY IHRDLATRNI LVENENRVKI
GDFGLTKVLP QDKEYYKVKE PGESPIFWYA PESLTESKFS VASDVWSFGV
VLYELFTYIE KSKSPPAEFM RMIGNDKQGQ MIVFHLIELL KNNGRLPRPD
GCPDEIYMIM TECWNNNVNQ RPSFRDLALR VDQIRDNMAG |
|
*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.
2. Protein Separation
Sample Preparation: 1 million cells from cell line K526 were lysed in an immuno-precipitation buffer containing 20 mM Tris-HCL (pH 7.6), 150 mM NaCl, 1mN EDTA, 0.1% NP-40, 100ug/ml PMSF, and 1mM sodium vanadate. Cell lysates were vortexed on ice for 30 minutes and spun to clear. The Supernatant was then incubated with protein A, and then with JAK2 specific antibody that has already been bound to protein A. Samples were incubated overnight, washed and then the beads were boiled in gel loading buffer and run for 1 hour on an SDS page gel (12% Bis-Tris). Resulting gel bands were then excised, reduced, alkylated and digested overnight with trypsin.
3. LC-MS/MS Data
NILVENENR
No LC-MS/MS for this peptide.
FGSLDTYLK
No LC-MS/MS for this peptide.
SNLLVFR
No LC-MS/MS for this peptide.
LSDPGISITVLPK
No LC-MS/MS for this peptide.
SDNIIFQFTK
No LC-MS/MS for this peptide.
IQDYHILTR
No LC-MS/MS for this peptide.
4. LC-MRM Screening
Peptides screening: Peptides and transitions for analysis were selected from previous LC-MS/MS experiments. The results were screened for those with the highest specificity and the most intense transitions.
