1. Basic Information

Name:	IGHG3_HUMAN
Accession#:	P01860
Description:	Ig gamma-3 chain C region
AA Number:	377
Sequence:	1 51 101 151 201 251 301 351    ASTKGPSVFP LAPCSRSTSG GTAALGCLVK DYFPEPVTVS WNSGALTSGV HTFPAVLQSS GLYSLSSVVT VPSSSLGTQT YTCNVNHKPS NTKVDKRVEL KTPLGDTTHT CPRCPEPKSC DTPPPCPRCP EPKSCDTPPP CPRCPEPKSC DTPPPCPRCP APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED PEVQFKWYVD GVEVHNAKTK PREEQYNSTF RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKTK GQPREPQVYT LPPSREEMTK NQVSLTCLVK GFYPSDIAVE WESSGQPENN YNTTPPMLDS DGSFFLYSKL TVDKSRWQQG NIFSCSVMHE ALHNRFTQKS LSLSPGK

*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.

2. Protein Separation

Sample Preparation: Human Serum was digested overnight with trypsin after reduction and alkylation with TCEP and IAA. Sample was then diluted 1:10 for analysis on a Thermo Scientific TSQ mass spectrometer

 

3. LC-MS/MS Data

VVSVLTVLHQDWLNGK

 

EPQVYTLPPSR

 

WYVDGVEVHNAK

 

ALPAPIEK

 

4. LC-MRM Screening

Peptides screening: Peptides and transitions for analysis were selected from previous LC-MS/MS experiments. The results were screened for those with the highest specificity and the most intense transitions.