1. Basic Information

Name:	IGHE_HUMAN
Accession#:	P01854
Description:	Ig epsilon chain C region
AA Number:	428
Sequence:	1 51 101 151 201 251 301 351 401    ASTQSPSVFP LTRCCKNIPS NATSVTLGCL ATGYFPEPVM VTWDTGSLNG TTMTLPATTL TLSGHYATIS LLTVSGAWAK QMFTCRVAHT PSSTDWVDNK TFSVCSRDFT PPTVKILQSS CDGGGHFPPT IQLLCLVSGY TPGTINITWL EDGQVMDVDL STASTTQEGE LASTQSELTL SQKHWLSDRT YTCQVTYQGH TFEDSTKKCA DSNPRGVSAY LSRPSPFDLF IRKSPTITCL VVDLAPSKGT VNLTWSRASG KPVNHSTRKE EKQRNGTLTV TSTLPVGTRD WIEGETYQCR VTHPHLPRAL MRSTTKTSGP RAAPEVYAFA TPEWPGSRDK RTLACLIQNF MPEDISVQWL HNEVQLPDAR HSTTQPRKTK GSGFFVFSRL EVTRAEWEQK DEFICRAVHE AASPSQTVQR AVSVNPGK

*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.

2. Protein Separation

Sample Preparation: Human Serum from an IgE multiple myeloma patient was digested overnight with trypsin after reduction and alkylation with TCEP and IAA. Sample was then diluted 1:10 for analysis on a Thermo Scientific TSQ mass spectrometer

 

3. LC-MS/MS Data

GSGFFVFSR

    No LC-MS/MS for this peptide.

VAHTPSSTDWVDNK

    No LC-MS/MS for this peptide.

HWLSDR

    No LC-MS/MS for this peptide.

DFTPPTVK

    No LC-MS/MS for this peptide.

VTHPHLPR

    No LC-MS/MS for this peptide.

AAPEVYAFATPEWPGSR

    No LC-MS/MS for this peptide.

4. LC-MRM Screening

Peptides screening: Peptides and transitions for analysis were selected using SRM builder, a peptide predictive program. The results were screened for those with the highest specificity and the most intense transitions.