1. Basic Information

Name:	IGHD_HUMAN
Accession#:	P01880
Description:	Ig delta chain C region
AA Number:	384
Sequence:	1 51 101 151 201 251 301 351    APTKAPDVFP IISGCRHPKD NSPVVLACLI TGYHPTSVTV TWYMGTQSQP QRTFPEIQRR DSYYMTSSQL STPLQQWRQG EYKCVVQHTA SKSKKEIFRW PESPKAQASS VPTAQPQAEG SLAKATTAPA TTRNTGRGGE EKKKEKEKEE QEERETKTPE CPSHTQPLGV YLLTPAVQDL WLRDKATFTC FVVGSDLKDA HLTWEVAGKV PTGGVEEGLL ERHSNGSQSQ HSRLTLPRSL WNAGTSVTCT LNHPSLPPQR LMALREPAAQ APVKLSLNLL ASSDPPEAAS WLLCEVSGFS PPNILLMWLE DQREVNTSGF APARPPPQPG STTFWAWSVL RVPAPPSPQP ATYTCVVSHE DSRTLLNASR SLEVSYVTDH GPMK

*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.

2. Protein Separation

Sample Preparation: Human Serum was digested overnight with trypsin after reduction and alkylation with TCEP and IAA. Sample was then diluted 1:10 for analysis on a Thermo Scientific TSQ mass spectrometer

 

3. LC-MS/MS Data

EPAAQAPVK

    No LC-MS/MS for this peptide.

VPTGGVEEGLLER

    No LC-MS/MS for this peptide.

4. LC-MRM Screening

Peptides screening: Peptides and transitions for analysis were selected using SRM builder, a peptide predictive program. The results were screened for those with the highest specificity and the most intense transitions.