1. Basic Information

Name:	IGHA2_HUMAN
Accession#:	P01877
Description:	Ig alpha-2 chain C region
AA Number:	340
Sequence:	1 51 101 151 201 251 301    ASPTSPKVFP LSLDSTPQDG NVVVACLVQG FFPQEPLSVT WSESGQNVTA RNFPPSQDAS GDLYTTSSQL TLPATQCPDG KSVTCHVKHY TNPSQDVTVP CPVPPPPPCC HPRLSLHRPA LEDLLLGSEA NLTCTLTGLR DASGATFTWT PSSGKSAVQG PPERDLCGCY SVSSVLPGCA QPWNHGETFT CTAAHPELKT PLTANITKSG NTFRPEVHLL PPPSEELALN ELVTLTCLAR GFSPKDVLVR WLQGSQELPR EKYLTWASRQ EPSQGTTTFA VTSILRVAAE DWKKGDTFSC MVGHEALPLA FTQKTIDRMA GKPTHVNVSV VMAEVDGTCY

*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.

2. Protein Separation

Sample Preparation: Human Serum was digested overnight with trypsin after reduction and alkylation with TCEP and IAA. Sample was then diluted 1:10 for analysis on a Thermo Scientific TSQ mass spectrometer

 

3. LC-MS/MS Data

DASGATFTWTPSSGK

    No LC-MS/MS for this peptide.

WLQGSQELPR

 

SAVQGPPER

 

4. LC-MRM Screening

Peptides screening: Peptides and transitions for analysis were selected from previous LC-MS/MS experiments. The results were screened for those with the highest specificity and the most intense transitions.