1. Basic Information

Name:	HSPB2_HUMAN
Accession#:	Q16082
Description:	Heat shock protein beta-2 A beta isoform
AA Number:	182
Sequence:	1 51 101 151    MSGRSVPHAH PATAEYEFAN PSRLGEQRFG EGLLPEEILT PTLYHGYYVR PRAAPAGEGS RAGASELRLS EGKFQAFLDV SHFTPDEVTV RTVDNLLEVS ARHPQRLDRH GFVSREFCRT YVLPADVDPW RVRAALSHDG ILNLEAPRGG RHLDTEVNEV YISLLPAPPD PEEEEEAAIV EP

*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.

2. Protein Separation

Sample Preparation: Cells from Myeloma cell line RPMI-8226 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. In solution digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. the equivilant of 50000 cells was was then analyzed on a Thermo Scientific TSQ mass spectrometer.

 

3. LC-MS/MS Data

TVDNLLEVSAR

    No LC-MS/MS for this peptide.

4. LC-MRM Screening

Peptides screening: Peptides and transitions for analysis were selected using a predictive peptide program. The results were screened for those with the highest specificity and the most intense transitions.