1. Basic Information

Name:	HSPB1_HUMAN
Accession#:	P04792
Description:	Heat shock protein beta-1
AA Number:	205
Sequence:	1 51 101 151 201    MTERRVPFSL LRGPSWDPFR DWYPHSRLFD QAFGLPRLPE EWSQWLGGSS WPGYVRPLPP AAIESPAVAA PAYSRALSRQ LSSGVSEIRH TADRWRVSLD VNHFAPDELT VKTKDGVVEI TGKHEERQDE HGYISRCFTR KYTLPPGVDP TQVSSSLSPE GTLTVEAPMP KLATQSNEIT IPVTFESRAQ LGGPEAAKSD ETAAK

*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.

2. Protein Separation

Sample Preparation: Cells from AML line U937 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The lysate was digested insolution with trypsin after reduction and alkylation with TCEP and IAA. ~50,000 cell equivilant of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.

 

3. LC-MS/MS Data

VSLDVNHFAPDELTVK

 

LATQSNEITIPVTFESR

 

LFDQAFGLPR

 

GPSWDPFR

    No LC-MS/MS for this peptide.

VPFSLLR

 

DWYPHSR

    No LC-MS/MS for this peptide.

QLSSGVSEIR

 

DGVVEITGK

 

4. LC-MRM Screening

Peptides screening: Peptides and transitions for analysis were selected using a predictive peptide program. The results were screened for those with the highest specificity and the most intense transitions.