1. Basic Information
| Name: | HRK_HUMAN | |||
| Accession#: | O00198 | |||
| Description: | Activator of apoptosis harakiri | |||
| AA Number: | 91 | |||
| Sequence: |
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*Green background amino acids are PTMs.
2. Protein Separation
Sample Preparation: Cells from 8226 cells were lysed in RIPA buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.
3. LC-MS/MS Data
ALGDELHQR
No LC-MS/MS for this peptide.
4. LC-MRM Screening
Peptides screening: Peptides and transitions for analysis were selected using a predictive peptide program. The results were screened for those with the highest specificity and the most intense transitions.
