1. Basic Information

Name:	H90B3_HUMAN
Accession#:	Q58FF7
Description:	Heat shock protein HSP 90-beta-3
AA Number:	597
Sequence:	1 51 101 151 201 251 301 351 401 451 501 551    MPEEVHHGEE EVETFAFQAE IAQLISLIIN TFYSNEEIFL QELISNASDA LDKIRYESLT DPSKLDSGKE LKIDIIPNPQ ERTLALVDTG IGMTKADLIN NLRTIAKSGT KACMEALQAE KLVVITKHND DEQYAWESSA GGSFTVHADH GEPIGRGTKV ILHLKEDQTE YLEERRVKEV VKKHSQFIGY PITLYLEKEQ DKEISDDEAE EEKGEKEEED KDDEEKPKIK DVGSDEEDDS KEYGEFYKSL TSDWEDHLAV KHFSVEGQLE FRALLFSPRR APFDLFENKK KKNNIKLYVR RVFIMDSCDE LIPEYLNFIH GVVDSEDLPL NISREMLQQS KILKYVSHMK ETQKSTYYIT GESKEQVANS AFVERVRKQG FEVVYMTEPI DEYCVQQLKE FDGKSLVSVT KEGLELPEDE EEKKKMEESK EKFENLCKLM KEILDKKVEK VTISNRLVSS PCCIVTSTYG WTANMEQIMK AQALRDNSTM GYMMAKKHLE INPDHPIMET LRQKAEADKN DKAVKDLVVL LFETALLSSG FSLEDPQTHS NHIYHMIKLG LGTDEDEVAA EEPSDAVPDE IPPLEGDEDA SRMEEVD

*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.

2. Protein Separation

Sample Preparation: Cells from AML line U937 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.

 

3. LC-MS/MS Data

ADLINNLR

    No LC-MS/MS for this peptide.

4. LC-MRM Screening

Peptides screening: Peptides and transitions for analysis were selected using a predictive peptide program. The results were screened for those with the highest specificity and the most intense transitions.