1. Basic Information

Name:	H90B2_HUMAN
Accession#:	Q58FF8
Description:	Heat shock protein HSP 90-beta 2
AA Number:	381
Sequence:	1 51 101 151 201 251 301 351    MPEEVHLGEK EVETFAFQAE IAQLMSLIIN TFYSNKEIFL WELISNASDA LDKIRYESLT DPSKLDSGKE LKIDIIPNTQ EHTLTLVDTG IGMTKADLIN NLGTIAKFQD QTEYLEEMQV KEVVEKHSQF LGYPITLYLE KEREKEISDG KAEEEKGEKE EENKDDEEKP KIEDVGSDEE DDSGKDKKKK TKKIKEKYID QEELNKTKPI WTRNTEDITQ EEYGEFYKSL TNDWKDHLAV RYFSVEEYVS RMKEIQKSIY YITGESKEQV ANSAFVEQVW KRDSRVVYMT EPIDGYQLKE FDGKSLVSVT KEGLELPEDG EEKKRMEERK AKFENLCKFM KETLDKKVEM VTVSNRLVSS SCCIVTSTYS WTANMEQIMK A

*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.

2. Protein Separation

Sample Preparation: Cells from AML line U937 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.

 

3. LC-MS/MS Data

SIYYITGESK

    No LC-MS/MS for this peptide.

YESLTDPSK

    No LC-MS/MS for this peptide.

ADLINNLGTIAK

    No LC-MS/MS for this peptide.

HSQFLGYPITLYLEK

    No LC-MS/MS for this peptide.

4. LC-MRM Screening

Peptides screening: Peptides and transitions for analysis were selected using a predictive peptide program. The results were screened for those with the highest specificity and the most intense transitions.