1. Basic Information

Name:	GRP78_HUMAN
Accession#:	P11021
Description:	78 kDa glucose-regulated protein
AA Number:	654
Sequence:	1 51 101 151 201 251 301 351 401 451 501 551 601 651    MKLSLVAAML LLLSAARAEE EDKKEDVGTV VGIDLGTTYS CVGVFKNGRV EIIANDQGNR ITPSYVAFTP EGERLIGDAA KNQLTSNPEN TVFDAKRLIG RTWNDPSVQQ DIKFLPFKVV EKKTKPYIQV DIGGGQTKTF APEEISAMVL TKMKETAEAY LGKKVTHAVV TVPAYFNDAQ RQATKDAGTI AGLNVMRIIN EPTAAAIAYG LDKREGEKNI LVFDLGGGTF DVSLLTIDNG VFEVVATNGD THLGGEDFDQ RVMEHFIKLY KKKTGKDVRK DNRAVQKLRR EVEKAKRALS SQHQARIEIE SFYEGEDFSE TLTRAKFEEL NMDLFRSTMK PVQKVLEDSD LKKSDIDEIV LVGGSTRIPK IQQLVKEFFN GKEPSRGINP DEAVAYGAAV QAGVLSGDQD TGDLVLLDVC PLTLGIETVG GVMTKLIPRN TVVPTKKSQI FSTASDNQPT VTIKVYEGER PLTKDNHLLG TFDLTGIPPA PRGVPQIEVT FEIDVNGILR VTAEDKGTGN KNKITITNDQ NRLTPEEIER MVNDAEKFAE EDKKLKERID TRNELESYAY SLKNQIGDKE KLGGKLSSED KETMEKAVEE KIEWLESHQD ADIEDFKAKK KELEEIVQPI ISKLYGSAGP PPTGEEDTAE KDEL

*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.

2. Protein Separation

Sample Preparation: 1 million cells from cell line 8226 were lysed in 8M urea in 30mM ammonium bicarbonate. The resulting lysate was reduced and alkalated prior to digestion overnight with trypsin. Digests were diluted and run with LC-MRM

 

3. LC-MS/MS Data

TFAPEEISAMVLTK

 

NQLTSNPENTVFDAK

 

ELEEIVQPIISK

 

SQIFSTASDNQPTVTIK

 

TWNDPSVQQDIK

 

VTHAVVTVPAYFNDAQR

 

ITPSYVAFTPEGER

 

VEIIANDQGNR

 

4. LC-MRM Screening

Peptides screening: Peptides and transitions for analysis were selected from previous LC-MS/MS experiments. The results were screened for those with the highest specificity and the most intense transitions.