1. Basic Information
| Name: | FANCM_HUMAN |
| Accession#: | Q8IYD8 |
| Description: | Fanconi anemia group M protein |
| AA Number: | 2048 |
| Sequence: |
1
51
101
151
201
251
301
351
401
451
501
551
601
651
701
751
801
851
901
951
1001
1051
1101
1151
1201
1251
1301
1351
1401
1451
1501
1551
1601
1651
1701
1751
1801
1851
1901
1951
2001
|
|
MSGRQRTLFQ TWGSSISRSS GTPGCSSGTE RPQSPGSSKA PLPAAAEAQL
ESDDDVLLVA AYEAERQLCL ENGGFCTSAG ALWIYPTNCP VRDYQLHISR
AALFCNTLVC LPTGLGKTFI AAVVMYNFYR WFPSGKVVFM APTKPLVTQQ
IEACYQVMGI PQSHMAEMTG STQASTRKEI WCSKRVLFLT PQVMVNDLSR
GACPAAEIKC LVIDEAHKAL GNYAYCQVVR ELVKYTNHFR ILALSATPGS
DIKAVQQVIT NLLIGQIELR SEDSPDILTY SHERKVEKLI VPLGEELAAI
QKTYIQILES FARSLIQRNV LMRRDIPNLT KYQIILARDQ FRKNPSPNIV
GIQQGIIEGE FAICISLYHG YELLQQMGMR SLYFFLCGIM DGTKGMTRSK
NELGRNEDFM KLYNHLECMF ARTRSTSANG ISAIQQGDKN KKFVYSHPKL
KKLEEVVIEH FKSWNAENTT EKKRDETRVM IFSSFRDSVQ EIAEMLSQHQ
PIIRVMTFVG HASGKSTKGF TQKEQLEVVK QFRDGGYNTL VSTCVGEEGL
DIGEVDLIIC FDSQKSPIRL VQRMGRTGRK RQGRIVIILS EGREERIYNQ
SQSNKRSIYK AISSNRQVLH FYQRSPRMVP DGINPKLHKM FITHGVYEPE
KPSRNLQRKS SIFSYRDGMR QSSLKKDWFL SEEEFKLWNR LYRLRDSDEI
KEITLPQVQF SSLQNEENKP AQESTTGIHQ LSLSEWRLWQ DHPLPTHQVD
HSDRCRHFIG LMQMIEGMRH EEGECSYELE VESYLQMEDV TSTFIAPRNE
SNNLASDTFI THKKSSFIKN INQGSSSSVI ESDEECAEIV KQTHIKPTKI
VSLKKKVSKE IKKDQLKKEN NHGIIDSVDN DRNSTVENIF QEDLPNDKRT
SDTDEIAATC TINENVIKEP CVLLTECQFT NKSTSSLAGN VLDSGYNSFN
DEKSVSSNLF LPFEEELYIV RTDDQFYNCH SLTKEVLANV ERFLSYSPPP
LSGLSDLEYE IAKGTALENL LFLPCAEHLR SDKCTCLLSH SAVNSQQNLE
LNSLKCINYP SEKSCLYDIP NDNISDEPSL CDCDVHKHNQ NENLVPNNRV
QIHRSPAQNL VGENNHDVDN SDLPVLSTDQ DESLLLFEDV NTEFDDVSLS
PLNSKSESLP VSDKTAISET PLVSQFLISD ELLLDNNSEL QDQITRDANS
FKSRDQRGVQ EEKVKNHEDI FDCSRDLFSV TFDLGFCSPD SDDEILEHTS
DSNRPLDDLY GRYLEIKEIS DANYVSNQAL IPRDHSKNFT SGTVIIPSNE
DMQNPNYVHL PLSAAKNEEL LSPGYSQFSL PVQKKVMSTP LSKSNTLNSF
SKIRKEILKT PDSSKEKVNL QRFKEALNST FDYSEFSLEK SKSSGPMYLH
KSCHSVEDGQ LLTSNESEDD EIFRRKVKRA KGNVLNSPED QKNSEVDSPL
HAVKKRRFPI NRSELSSSDE SENFPKPCSQ LEDFKVCNGN ARRGIKVPKR
QSHLKHVARK FLDDEAELSE EDAEYVSSDE NDESENEQDS SLLDFLNDET
QLSQAINDSE MRAIYMKSLR SPMMNNKYKM IHKTHKNINI FSQIPEQDET
YLEDSFCVDE EESCKGQSSE EEVCVDFNLI TDDCFANSKK YKTRRAVMLK
EMMEQNCAHS KKKLSRIILP DDSSEEENNV NDKRESNIAV NPSTVKKNKQ
QDHCLNSVPS GSSAQSKVRS TPRVNPLAKQ SKQTSLNLKD TISEVSDFKP
QNHNEVQSTT PPFTTVDSQK DCRKFPVPQK DGSALEDSST SGASCSKSRP
HLAGTHTSLR LPQEGKGTCI LVGGHEITSG LEVISSLRAI HGLQVEVCPL
NGCDYIVSNR MVVERRSQSE MLNSVNKNKF IEQIQHLQSM FERICVIVEK
DREKTGDTSR MFRRTKSYDS LLTTLIGAGI RILFSSCQEE TADLLKELSL
VEQRKNVGIH VPTVVNSNKS EALQFYLSIP NISYITALNM CHQFSSVKRM
ANSSLQEISM YAQVTHQKAE EIYRYIHYVF DIQMLPNDLN QDRLKSDI |
|
*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.
2. Protein Separation
Sample Preparation: Cells from line 8226 were lysed in RIPA buffer. The equivalent of 50,000 cells were loaded onto an SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.
3. LC-MS/MS Data
SSIFSYR
No LC-MS/MS for this peptide.
STSANGISAIQQGDK
No LC-MS/MS for this peptide.
DYQLHISR
No LC-MS/MS for this peptide.
ELSLVEQR
No LC-MS/MS for this peptide.
IVIILSEGR
No LC-MS/MS for this peptide.
LEEVVIEHFK
No LC-MS/MS for this peptide.
FVYSHPK
No LC-MS/MS for this peptide.
4. LC-MRM Screening
Peptides screening: Peptides and transitions for analysis were selected using a predictive peptide program. The results were screened for those with the highest specificity and the most intense transitions.
