1. Basic Information
| Name: | FANCI_HUMAN |
| Accession#: | Q9NVI1 |
| Description: | Fanconi anemia group I protein |
| AA Number: | 1328 |
| Sequence: |
1
51
101
151
201
251
301
351
401
451
501
551
601
651
701
751
801
851
901
951
1001
1051
1101
1151
1201
1251
1301
|
|
MDQKILSLAA EKTADKLQEF LQTLREGDLT NLLQNQAVKG KVAGALLRAI
FKGSPCSEEA GTLRRRKIYT CCIQLVESGD LQKEIASEII GLLMLEAHHF
PGPLLVELAN EFISAVREGS LVNGKSLELL PIILTALATK KENLAYGKGV
LSGEECKKQL INTLCSGRWD QQYVIQLTSM FKDVPLTAEE VEFVVEKALS
MFSKMNLQEI PPLVYQLLVL SSKGSRKSVL EGIIAFFSAL DKQHNEEQSG
DELLDVVTVP SGELRHVEGT IILHIVFAIK LDYELGRELV KHLKVGQQGD
SNNNLSPFSI ALLLSVTRIQ RFQDQVLDLL KTSVVKSFKD LQLLQGSKFL
QNLVPHRSYV STMILEVVKN SVHSWDHVTQ GLVELGFILM DSYGPKKVLD
GKTIETSPSL SRMPNQHACK LGANILLETF KIHEMIRQEI LEQVLNRVVT
RASSPISHFL DLLSNIVMYA PLVLQSCSSK VTEAFDYLSF LPLQTVQRLL
KAVQPLLKVS MSMRDCLILV LRKAMFANQL DARKSAVAGF LLLLKNFKVL
GSLSSSQCSQ SLSVSQVHVD VHSHYNSVAN ETFCLEIMDS LRRCLSQQAD
VRLMLYEGFY DVLRRNSQLA NSVMQTLLSQ LKQFYEPKPD LLPPLKLEAC
ILTQGDKISL QEPLDYLLCC IQHCLAWYKN TVIPLQQGEE EEEEEEAFYE
DLDDILESIT NRMIKSELED FELDKSADFS QSTSIGIKNN ICAFLVMGVC
EVLIEYNFSI SSFSKNRFED ILSLFMCYKK LSDILNEKAG KAKTKMANKT
SDSLLSMKFV SSLLTALFRD SIQSHQESLS VLRSSNEFMR YAVNVALQKV
QQLKETGHVS GPDGQNPEKI FQNLCDITRV LLWRYTSIPT SVEESGKKEK
GKSISLLCLE GLQKIFSAVQ QFYQPKIQQF LRALDVTDKE GEEREDADVS
VTQRTAFQIR QFQRSLLNLL SSQEEDFNSK EALLLVTVLT SLSKLLEPSS
PQFVQMLSWT SKICKENSRE DALFCKSLMN LLFSLHVSYK SPVILLRDLS
QDIHGHLGDI DQDVEVEKTN HFAIVNLRTA APTVCLLVLS QAEKVLEEVD
WLITKLKGQV SQETLSEEAS SQATLPNQPV EKAIIMQLGT LLTFFHELVQ
TALPSGSCVD TLLKDLCKMY TTLTALVRYY LQVCQSSGGI PKNMEKLVKL
SGSHLTPLCY SFISYVQNKS KSLNYTGEKK EKPAAVATAM ARVLRETKPI
PNLIFAIEQY EKFLIHLSKK SKVNLMQHMK LSTSRDFKIK GNILDMVLRE
DGEDENEEGT ASEHGGQNKE PAKKKRKK |
|
*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.
2. Protein Separation
Sample Preparation: Cells from 8226 were lysed in RIPA buffer. The equivalent of 100,000 cells were loaded onto an SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.
3. LC-MS/MS Data
SELEDFELDK
No LC-MS/MS for this peptide.
AVQPLLK
No LC-MS/MS for this peptide.
TNHFAIVNLR
No LC-MS/MS for this peptide.
YAVNVALQK
No LC-MS/MS for this peptide.
FLIHLSK
No LC-MS/MS for this peptide.
LSDILNEK
No LC-MS/MS for this peptide.
YTSIPTSVEESGK
No LC-MS/MS for this peptide.
VAGALLR
No LC-MS/MS for this peptide.
FQDQVLDLLK
QEILEQVLNR
No LC-MS/MS for this peptide.
TEITSPSLSR
No LC-MS/MS for this peptide.
TIETSPSLSR
No LC-MS/MS for this peptide.
EGDLTNLLQNQAVK
No LC-MS/MS for this peptide.
SPVILLR
No LC-MS/MS for this peptide.
IFSAVQQFYQPK
No LC-MS/MS for this peptide.
DLQLLQGSK
No LC-MS/MS for this peptide.
LDYELGR
No LC-MS/MS for this peptide.
4. LC-MRM Screening
Peptides screening: Peptides and transitions for analysis were selected using a predictive peptide program. The results were screened for those with the highest specificity and the most intense transitions.
