1. Basic Information

Name:	DVL1L_HUMAN
Accession#:	P54792
Description:	Segment polarity protein dishevelled homolog DVL-1-like
AA Number:	670
Sequence:	1 51 101 151 201 251 301 351 401 451 501 551 601 651    MAETKIIYHM DEEETPYLVK LPVAPERVTL ADFKNVLSNR PVHAYKFFFK SMDQDFGVVK EEIFDDNAKL PCFNGRVVSW LVLVEGAHSD AGSQGTDSHT DLPPPLERTG GIGDSRSPSF QPDVASSRDG MDNETGTESM VSHRRDRARR RNREEAARTN GHPRGDRRRD VGLPPDSAST ALSSELESSS FVDSDEDDST SRLSSSTEQS TSSRLIRKHK RRRRKQRLRQ ADRASSFSSM TDSTMSLNII TVTLNMERHH FLGICIVGQS NDRGDGGIYI GSIMKGGAVA ADGRIEPGDM LLQVNDVNFE NMSNDDAVRV LREIVSQTGP ISLTVAKCWD PTPRSYFTVP RPDPVRPIDP AAWLSHTAAL TGALPRPQLE EAPLTVESDM NTVVRVMQLP DSGLEIRDRM WLKITIANAV IGADVVDWLY THVEGFKERR EARKYASSLL KHGFLRHTVN KITFSEQCYY VFGDLCSNLA TLNLNSGSSG TSDQDTLAPL PHPAAPWPLG QGYPYQYPGP PPCFPPAYQD PGFSYGSGST GSQQSEGSKS SGSTRNTLRP PACEKERRAA GSGDSDSESD HTAPSGVGSS WRERPADQLS RGSSPRSQAS SYAPGLPPPH PTTKAYTVVG GPPGGPPVRE LAAVPPELTG SRQSFQKAMG NPCEFFVDIM

*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.

2. Protein Separation

Sample Preparation: Cells from colon caner line HCT 116 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.

 

3. LC-MS/MS Data

SPSFQPDVASSR

    No LC-MS/MS for this peptide.

4. LC-MRM Screening

Peptides screening: Peptides and transitions for analysis were selected using a predictive peptide program. The results were screened for those with the highest specificity and the most intense transitions.