1. Basic Information

Name:	CYTC_HUMAN
Accession#:	P01034
Description:	Cystatin-C
AA Number:	146
Sequence:	1 51 101    MAGPLRAPLL LLAILAVALA VSPAAGSSPG KPPRLVGGPM DASVEEEGVR RALDFAVGEY NKASNDMYHS RALQVVRARK QIVAGVNYFL DVELGRTTCT KTQPNLDNCP FHDQPHLKRK AFCSFQIYAV PWQGTMTLSK STCQDA

*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.

2. Protein Separation

Sample Preparation: Control urine was processed via ultrafiltration according to Moffitt Proteomics protocol and subjected to in-solution digestion with trypsin, following thermal denaturation, reduction, and alkylation.

 

3. LC-MS/MS Data

QIVAGVNYFLDVELGR

    No LC-MS/MS for this peptide.

ALDFAVGEYNK

    No LC-MS/MS for this peptide.

4. LC-MRM Screening

Peptides screening: Peptides were chosen in Skyline with preference given to tryptic sequences observed in the NIST Human LC-MS/MS library; for such peptides, the 5 most abundant y ions were automatically chosen. Additional peptides were chosen with preference given to sequences between 8 - 15 amino acids long, with no missed cleavages, and with no methionines. Fragment ions were chosen to represent the largest y ions, with attention to the presence of P, D, E, and H residues in the sequence.