1. Basic Information

Name:	CIR1_HUMAN
Accession#:	Q86X95
Description:	Corepressor interacting with RBPJ 1
AA Number:	450
Sequence:	1 51 101 151 201 251 301 351 401    MGKSFANFMC KKDFHPASKS NIKKVWMAEQ KISYDKKKQE ELMQQYLKEQ ESYDNRLLMG DERVKNGLNF MYEAPPGAKK ENKEKEETEG ETEYKFEWQK GAPREKYAKD DMNIRDQPFG IQVRNVRCIK CHKWGHVNTD RECPLFGLSG INASSVPTDG SGPSMHPSEL IAEMRNSGFA LKRNVLGRNL TANDPSQEYV ASEGEEDPEV EFLKSLTTKQ KQKLLRKLDR LEKKKKKKDR KKKKFQKSRS KHKKHKSSSS SSSSSSSSSS TETSESSSES ESNNKEKKIQ RKKRKKNKCS GHNNSDSEEK DKSKKRKLHE ELSSSHHNRE KAKEKPRFLK HESSREDSKW SHSDSDKKSR THKHSPEKRG SERKEGSSRS HGREERSRRS RSRSPGSYKQ RETRKRAQRN PGEEQSRRND SRSHGTDLYR GEKMYREHPG GTHTKVTQRE

*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.

2. Protein Separation

Sample Preparation: Cells from colon caner line HCT 116 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 60,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/6 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.

 

3. LC-MS/MS Data

NPGEEQSR

    No LC-MS/MS for this peptide.

DFHPASK

    No LC-MS/MS for this peptide.

4. LC-MRM Screening

Peptides screening: Peptides and transitions for analysis were selected using a predictive peptide program. The results were screened for those with the highest specificity and the most intense transitions.