1. Basic Information
| Name: | CH10_HUMAN | |||
| Accession#: | P61604 | |||
| Description: | 10 kDa heat shock protein, mitochondrial | |||
| AA Number: | 102 | |||
| Sequence: |
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*Green background amino acids are PTMs.
2. Protein Separation
Sample Preparation: Cells from line 8226 were lysed in 8M urea/100mM ammonium bicarbonate and then were subjected to in-solution digestion with trypsin following reduction and alkylation.
3. LC-MS/MS Data
GGEIQPVSVK
No LC-MS/MS for this peptide.
4. LC-MRM Screening
Peptides screening: Peptides were chosen in Skyline with preference given to tryptic sequences observed in the NIST Human LC-MS/MS library; for such peptides, the 5 most abundant y ions were automatically chosen. Additional peptides were chosen with preference given to sequences between 8 - 15 amino acids long, with no missed cleavages, and with no methionines. Fragment ions were chosen to represent the largest y ions, with attention to the presence of P, D, E, and H residues in the sequence.
