1. Basic Information
| Name: | BNIP3_HUMAN | |||
| Accession#: | Q12983 | |||
| Description: | BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 | |||
| AA Number: | 194 | |||
| Sequence: |
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*Green background amino acids are PTMs.
2. Protein Separation
Sample Preparation: Cells from line 8226 were lysed in RIPA buffer. The equivalent of 50,000 cells were loaded onto an SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.
3. LC-MS/MS Data
LTTSTSTF
No LC-MS/MS for this peptide.
SQTPQDTNR
No LC-MS/MS for this peptide.
4. LC-MRM Screening
Peptides screening: Peptides and transitions for analysis were selected with a peptide predictive program. The results were screened for those with the highest specificity and the most intense transitions.
