1. Basic Information

Name:	BCAS1_HUMAN
Accession#:	O75363
Description:	Breast carcinoma-amplified sequence 1
AA Number:	584
Sequence:	1 51 101 151 201 251 301 351 401 451 501 551    MGNQMSVPQR VEDQENEPEA ETYQDNASAL NGVPVVVSTH TVQHLEEVDL GISVKTDNVA TSSPETTEIS AVADANGKNL GKEAKPEAPA AKSRFFLMLS RPVPGRTGDQ AADSSLGSVK LDVSSNKAPA NKDPSESWTL PVAAGPGQDT DKTPGHAPAQ DKVLSAARDP TLLPPETGGA GGEAPSKPKD SSFFDKFFKL DKGQEKVPGD SQQEAKRAEH QDKVDEVPGL SGQSDDVPAG KDIVDGKEKE GQELGTADCS VPGDPEGLET AKDDSQAAAI AENNNSIMSF FKTLVSPNKA ETKKDPEDTG AEKSPTTSAD LKSDKANFTS QETQGAGKNS KGCNPSGHTQ SVTTPEPAKE GTKEKSGPTS LPLGKLFWKK SVKEDSVPTG AEENVVCESP VEIIKSKEVE SALQTVDLNE GDAAPEPTEA KLKREESKPR TSLMAFLRQM SVKGDGGITH SEEINGKDSS CQTSDSTEKT ITPPEPEPTG APQKGKEGSS KDKKSAAEMN KQKSNKQEAK EPAQCTEQAT VDTNSLQNGD KLQKRPEKRQ QSLGGFFKGL GPKRMLDAQV QTDPVSIGPV GKSK

*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.

2. Protein Separation

Sample Preparation: Cells from colon caner line HCT 116 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 60,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/6 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.

 

3. LC-MS/MS Data

ANFTSQETQGAGK

    No LC-MS/MS for this peptide.

4. LC-MRM Screening

Peptides screening: Peptides and transitions for analysis were selected using a predictive peptide program. The results were screened for those with the highest specificity and the most intense transitions.