1. Basic Information

Name:	BAX_HUMAN
Accession#:	Q07812
Description:	Apoptosis regulator BAX
AA Number:	192
Sequence:	1 51 101 151    MDGSGEQPRG GGPTSSEQIM KTGALLLQGF IQDRAGRMGG EAPELALDPV PQDASTKKLS ECLKRIGDEL DSNMELQRMI AAVDTDSPRE VFFRVAADMF SDGNFNWGRV VALFYFASKL VLKALCTKVP ELIRTIMGWT LDFLRERLLG WIQDQGGWDG LLSYFGTPTW QTVTIFVAGV LTASLTIWKK MG

*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.

2. Protein Separation

Sample Preparation: Cells from line 8226 were lysed in RIPA buffer. The equivalent of 50,000 cells were loaded onto an SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.

 

3. LC-MS/MS Data

VVALFYFASK

    No LC-MS/MS for this peptide.

TGALLLQGFIQDR

    No LC-MS/MS for this peptide.

4. LC-MRM Screening

Peptides screening: Peptides and transitions for analysis were selected using both LC-MS/MS data and a predictive peptide program. The results were screened for those with the highest specificity and the most intense transitions.