1. Basic Information
| Name: | ARAF_HUMAN | |||
| Accession#: | P10398 | |||
| Description: | Serine/threonine-protein kinase A-raf | |||
| AA Number: | 606 | |||
| Sequence: |
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*Green background amino acids are PTMs.
2. Protein Separation
Sample Preparation: Cells from Melanoma cancer lines were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 200,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/6 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.
3. LC-MS/MS Data
FLFHGFR
No LC-MS/MS for this peptide.
GYLSPDLSK
No LC-MS/MS for this peptide.
SASEPSLHR
No LC-MS/MS for this peptide.
GSPSPASVSSGR
No LC-MS/MS for this peptide.
DQIIFMVGR
No LC-MS/MS for this peptide.
DGMSVYDSLDK
No LC-MS/MS for this peptide.
IGTGSFGTVFR
No LC-MS/MS for this peptide.
IGDFGLATVK
No LC-MS/MS for this peptide.
QQFYHSVQDLSGGSR
No LC-MS/MS for this peptide.
EERPLFPQILATIELLQR
No LC-MS/MS for this peptide.
VSQPTAEQAQAFK
No LC-MS/MS for this peptide.
4. LC-MRM Screening
Peptides screening: Peptides and transitions for analysis were selected using a predictive peptide program. The results were screened for those with the highest specificity and the most intense transitions.
