1. Basic Information

Name:	ANXA1_HUMAN
Accession#:	P04083
Description:	Annexin A1
AA Number:	346
Sequence:	1 51 101 151 201 251 301    MAMVSEFLKQ AWFIENEEQE YVQTVKSSKG GPGSAVSPYP TFNPSSDVAA LHKAIMVKGV DEATIIDILT KRNNAQRQQI KAAYLQETGK PLDETLKKAL TGHLEEVVLA LLKTPAQFDA DELRAAMKGL GTDEDTLIEI LASRTNKEIR DINRVYREEL KRDLAKDITS DTSGDFRNAL LSLAKGDRSE DFGVNEDLAD SDARALYEAG ERRKGTDVNV FNTILTTRSY PQLRRVFQKY TKYSKHDMNK VLDLELKGDI EKCLTAIVKC ATSKPAFFAE KLHQAMKGVG TRHKALIRIM VSRSEIDMND IKAFYQKMYG ISLCQAILDE TKGDYEKILV ALCGGN

*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.

2. Protein Separation

Sample Preparation: Cells from colon caner line HCT 116 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 60,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/6 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.

 

3. LC-MS/MS Data

TPAQFDADELR

 

ALYEAGER

    No LC-MS/MS for this peptide.

SYPQLR

    No LC-MS/MS for this peptide.

GVDEATIIDILTK

    No LC-MS/MS for this peptide.

DITSDTSGDFR

 

VLDLELK

    No LC-MS/MS for this peptide.

GTDVNVFNTILTTR

 

GLGTDEDTLIEILASR

 

NALLSLAK

    No LC-MS/MS for this peptide.

SEDFGVNEDLADSDAR

 

4. LC-MRM Screening

Peptides screening: Peptides and transitions for analysis were selected using a predictive peptide program. The results were screened for those with the highest specificity and the most intense transitions.