1. Basic Information

Name:	AL1A1_HUMAN
Accession#:	P00352
Description:	Retinal dehydrogenase 1
AA Number:	501
Sequence:	1 51 101 151 201 251 301 351 401 451    MSSSGTPDLP VLLTDLKIQY TKIFINNEWH DSVSGKKFPV FNPATEEELC QVEEGDKEDV DKAVKAARQA FQIGSPWRTM DASERGRLLY KLADLIERDR LLLATMESMN GGKLYSNAYL NDLAGCIKTL RYCAGWADKI QGRTIPIDGN FFTYTRHEPI GVCGQIIPWN FPLVMLIWKI GPALSCGNTV VVKPAEQTPL TALHVASLIK EAGFPPGVVN IVPGYGPTAG AAISSHMDID KVAFTGSTEV GKLIKEAAGK SNLKRVTLEL GGKSPCIVLA DADLDNAVEF AHHGVFYHQG QCCIAASRIF VEESIYDEFV RRSVERAKKY ILGNPLTPGV TQGPQIDKEQ YDKILDLIES GKKEGAKLEC GGGPWGNKGY FVQPTVFSNV TDEMRIAKEE IFGPVQQIMK FKSLDDVIKR ANNTFYGLSA GVFTKDIDKA ITISSALQAG TVWVNCYGVV SAQCPFGGFK MSGNGRELGE YGFHEYTEVK TVTVKISQKN S

*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.

2. Protein Separation

Sample Preparation: Cells from ALDH+ sorted RH-CA Sphere cells were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 30,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.

 

3. LC-MS/MS Data

ILDLIESGK

 

ANNTFYGLSAGVFTK

 

VAFTGSTEVGK

    No LC-MS/MS for this peptide.

IFVEESIYDEFVR

    No LC-MS/MS for this peptide.

YILGNPLTPGVTQGPQIDK

    No LC-MS/MS for this peptide.

TIPIDGNFFTYTR

 

QAFQIGSPWR

    No LC-MS/MS for this peptide.

ELGEYGFHEYTEVK

    No LC-MS/MS for this peptide.

SLDDVIK

    No LC-MS/MS for this peptide.

IFINNEWHDSVSGK

 

4. LC-MRM Screening

Peptides screening: Peptides and transitions for analysis were selected using a predictive peptide program. The results were screened for those with the highest specificity and the most intense transitions.