1. Basic Information

Name:	AKT3_HUMAN
Accession#:	Q9Y243
Description:	RAC-gamma serine/threonine-protein kinase
AA Number:	479
Sequence:	1 51 101 151 201 251 301 351 401 451    MSDVTIVKEG WVQKRGEYIK NWRPRYFLLK TDGSFIGYKE KPQDVDLPYP LNNFSVAKCQ LMKTERPKPN TFIIRCLQWT TVIERTFHVD TPEEREEWTE AIQAVADRLQ RQEEERMNCS PTSQIDNIGE EEMDASTTHH KRKTMNDFDY LKLLGKGTFG KVILVREKAS GKYYAMKILK KEVIIAKDEV AHTLTESRVL KNTRHPFLTS LKYSFQTKDR LCFVMEYVNG GELFFHLSRE RVFSEDRTRF YGAEIVSALD YLHSGKIVYR DLKLENLMLD KDGHIKITDF GLCKEGITDA ATMKTFCGTP EYLAPEVLED NDYGRAVDWW GLGVVMYEMM CGRLPFYNQD HEKLFELILM EDIKFPRTLS SDAKSLLSGL LIKDPNKRLG GGPDDAKEIM RHSFFSGVNW QDVYDKKLVP PFKPQVTSET DTRYFDEEFT AQTITITPPE KYDEDGMDCM DNERRPHFPQ FSYSASGRE

*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.

2. Protein Separation

Sample Preparation: Cells from colon cancer line HT116 were lysed in 8M urea/100mM Ammonium bicarbonate buffer. The equivalent of 60,000 cells were loaded onto an SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/6 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.

 

3. LC-MS/MS Data

HPFLTSLK

    No LC-MS/MS for this peptide.

FYGAEIVSALDYLHSGK

    No LC-MS/MS for this peptide.

TFHVDTPEER

    No LC-MS/MS for this peptide.

SLLSGLLIK

    No LC-MS/MS for this peptide.

EEWTEAIQAVADR

    No LC-MS/MS for this peptide.

DEVAHTLTESR

    No LC-MS/MS for this peptide.

LENLMLDK

    No LC-MS/MS for this peptide.

TDGSFIGYK

    No LC-MS/MS for this peptide.

4. LC-MRM Screening

Peptides screening: Peptides and transitions for analysis were selected using a predictive peptide program. The results were screened for those with the highest specificity and the most intense transitions.