1. Basic Information

Name:	ACTC_HUMAN
Accession#:	P68032
Description:	Actin, alpha cardiac muscle 1
AA Number:	376
Sequence:	1 51 101 151 201 251 301 351    MCDDEETTAL VCDNGSGLVK AGFAGDDAPR AVFPSIVGRP RHQGVMVGMG QKDSYVGDEA SKRGILTLKY PIEHGIITNW DDMEKIWHHT FYNELRVAPE EHPTLLTEAP LNPKANREKM TQIMFETFNV PAMYVAIQAV LSLYASGRTT GIVLDSGDGV THNVPIYEGY ALPHAIMRLD LAGRDLTDYL MKILTERGYS FVTTAEREIV RDIKEKLCYV ALDFENEMAT AASSSSLEKS YELPDGQVIT IGNERFRCPE TLFQPSFIGM ESAGIHETTY NSIMKCDIDI RKDLYANNVL SGGTTMYPGI ADRMQKEITA LAPSTMKIKI IAPPERKYSV WIGGSILASL STFQQMWISK QEYDEAGPSI VHRKCF

*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.

2. Protein Separation

Sample Preparation: Cells from line 8226 were lysed in 8M urea/100mM ammonium bicarbonate and then were subjected to in-solution digestion with trypsin following reduction and alkylation.

 

3. LC-MS/MS Data

DSYVGDEAQSK

    No LC-MS/MS for this peptide.

4. LC-MRM Screening

Peptides screening: Peptides were chosen in Skyline with preference given to tryptic sequences observed in the NIST Human LC-MS/MS library; for such peptides, the 5 most abundant y ions were automatically chosen. Additional peptides were chosen with preference given to sequences between 8 - 15 amino acids long, with no missed cleavages, and with no methionines. Fragment ions were chosen to represent the largest y ions, with attention to the presence of P, D, E, and H residues in the sequence.