1. Basic Information

Name:	VIME_HUMAN
Accession#:	P08670
Description:	Vimentin
AA Number:	466
Sequence:	1 51 101 151 201 251 301 351 401 451    MSTRSVSSSS YRRMFGGPGT ASRPSSSRSY VTTSTRTYSL GSALRPSTSR SLYASSPGGV YATRSSAVRL RSSVPGVRLL QDSVDFSLAD AINTEFKNTR TNEKVELQEL NDRFANYIDK VRFLEQQNKI LLAELEQLKG QGKSRLGDLY EEEMRELRRQ VDQLTNDKAR VEVERDNLAE DIMRLREKLQ EEMLQREEAE NTLQSFRQDV DNASLARLDL ERKVESLQEE IAFLKKLHEE EIQELQAQIQ EQHVQIDVDV SKPDLTAALR DVRQQYESVA AKNLQEAEEW YKSKFADLSE AANRNNDALR QAKQESTEYR RQVQSLTCEV DALKGTNESL ERQMREMEEN FAVEAANYQD TIGRLQDEIQ NMKEEMARHL REYQDLLNVK MALDIEIATY RKLLEGEESR ISLPLPNFSS LNLRETNLDS LPLVDTHSKR TLLIKTVETR DGQVINETSQ HHDDLE

*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.

2. Protein Separation

Sample Preparation: Cells from the breast cancer cell line HS578T were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.

 

3. LC-MS/MS Data

QQYESVAAK

 

FANYIDK

 

QDVDNASLAR

 

QVDQLTNDK

    No LC-MS/MS for this peptide.

FADLSEAANR

 

VESLQEEIAFLK

    No LC-MS/MS for this peptide.

ETNLDSLPLVDTHSK

    No LC-MS/MS for this peptide.

EEAENTLQSFR

 

ILLAELEQLK

 

SSVPGVR

    No LC-MS/MS for this peptide.

LLQDSVDFSLADAINTEFK

 

SYVTTSTR

 

DGQVINETSQHHDDLE

 

SLYASSPGGVYATR

 

ISLPLPNFSSLNLR

 

TYSLGSALRPSTSR

    No LC-MS/MS for this peptide.

4. LC-MRM Screening

Peptides screening: Peptides and transitions for analysis were selected using a predictive peptide program. The results were screened for those with the highest specificity and the most intense transitions.