1. Basic Information

Name:	AKT2_HUMAN
Accession#:	P31751
Description:	RAC-Beta serine/threonine-protein kinase
AA Number:	480
Sequence:	1 51 101 151 201 251 301 351 401 451    MNEVSVIKEG WLHKRGEYIK TWRPRYFLLK SDGSFIGYKE RPEAPDQTLP PLNNFSVAEC QLMKTERPRP NTFVIRCLQW TTVIERTFHV DSPDEREEWM RAIQMVANSL KQRAPGEDPM DYKCGSPSDS STTEEMEVAV SKARAKVTMN DFDYLKLLGK GTFGKVILVR EKATGRYYAM KILRKEVIIA KDEVAHTVTE SRVLQNTRHP FLTALKYAFQ THDRLCFVME YANGGELFFH LSRERVFTEE RARFYGAEIV SALEYLHSRD VVYRDIKLEN LMLDKDGHIK ITDFGLCKEG ISDGATMKTF CGTPEYLAPE VLEDNDYGRA VDWWGLGVVM YEMMCGRLPF YNQDHERLFE LILMEEIRFP RTLSPEAKSL LAGLLKKDPK QRLGGGPSDA KEVMEHRFFL SINWQDVVQK KLLPPFKPQV TSEVDTRYFD DEFTAQSITI TPPDRYDSLG LLELDQRTHF PQFSYSASIR

*Highlighted peptides (with yellow background) have developed assays.
*Green background amino acids are PTMs.

2. Protein Separation

Sample Preparation: Cells from colon cancer line HT116 were lysed in 8M urea/100mM Ammonium bicarbonate buffer. The equivalent of 60,000 cells were loaded onto an SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/6 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.

 

3. LC-MS/MS Data

TLSPEAK

    No LC-MS/MS for this peptide.

VFTEER

    No LC-MS/MS for this peptide.

SLLAGLLK

    No LC-MS/MS for this peptide.

THFPQFSYSASIR

    No LC-MS/MS for this peptide.

TFHVDSPDER

    No LC-MS/MS for this peptide.

SDGSFIGYK

    No LC-MS/MS for this peptide.

HPFLTALK

    No LC-MS/MS for this peptide.

LLPPFKPQVTSEVDTR

    No LC-MS/MS for this peptide.

TERPRPNTFVIR

    No LC-MS/MS for this peptide.

LENLMLDK

    No LC-MS/MS for this peptide.

FYGAEIVSALEYLHSR

    No LC-MS/MS for this peptide.

4. LC-MRM Screening

Peptides screening: Peptides and transitions for analysis were selected using a predictive peptide program. The results were screened for those with the highest specificity and the most intense transitions.