Information for Peptide: YYTPVLAK from STA5A_HUMAN

1. Biological Peptide

1.1 Native Sequence

    K.YYTPVLAK.A

1.2 Peptide Properties

Start	Stop	MW	m/z for 2+	m/z for 3+	pI
682	689	953.52	477.77	318.85	8.5

1.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
Y	b		y
Y	b		y
T	b		y
P	b		y
V	b		y
L	b		y
A	b		y
K	b		y

2. Synthetic Peptide

2.1 Synthetic (Internal Standard) Peptide Sequence

YYTPVL(C6N1)AK (Modification: Stable Isotope on L6 (U-13C6, N15) )

2.2 Peptide Properties

MW	m/z for 2+	m/z for 3+	pI
960.54	481.28	321.19	8.5

2.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
Y	b		y
Y	b		y
T	b		y
P	b		y
V	b		y
L	b		y
A	b		y
K	b		y

2.4 Peptide Synthesis Report

Click to view/download synthesis report for YYTPVLAK

2.5 QqQ MS/MS

Transition Table:

Transition	m/z of Transition	Collision Energy
y4	437.302	20.0
y5	534.355	20.0
y6	635.403	20.0
y7	798.466	20.0

2.6 Calibration Curve of Standard

Description: NONE

 

Experiment Description: 1 million cells from cell line K526 were lysed in an immuno-precipitation buffer containing 20 mM Tris-HCL (pH 7.6), 150 mM NaCl, 1mN EDTA, 0.1% NP-40, 100ug/ml PMSF, and 1mM sodium vanadate. Cell lysates were vortexed on ice for 30 minutes and spun to clear. The Supernatant was then incubated with protein A, and then with ABL1 specific antibody that has already been bound to protein A. Samples were incubated overnight, washed and then the beads were boiled in gel loading buffer and run for 1 hour on an SDS page gel (12% Bis-Tris). Resulting gel bands were then excised, reduced, alkylated and digested overnight with trypsin. Internal standard was injected concurrently

2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times

2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides