Information for Peptide: YSFLQFDPAPR from PP2AA_HUMAN
1. Biological Peptide
1.1 Native Sequence
K.YSFLQFDPAPR.R
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 284 | 294 | 1339.66 | 670.84 | 447.56 | 5.84 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| Y | b | y | ||
| S | b | y | ||
| F | b | y | ||
| L | b | y | ||
| Q | b | y | ||
| F | b | y | ||
| D | b | y | ||
| P | b | y | ||
| A | b | y | ||
| P | b | y | ||
| R | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
YSFLQFDPAP(C5N1)R (Modification: Stable Isotope on P10 (U-13C5, 15N) )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 1345.67 | 673.84 | 449.56 | 5.84 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| Y | b | y | ||
| S | b | y | ||
| F | b | y | ||
| L | b | y | ||
| Q | b | y | ||
| F | b | y | ||
| D | b | y | ||
| P | b | y | ||
| A | b | y | ||
| P | b | y | ||
| R | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for YSFLQFDPAPR
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y3 | 349.21 | 26.0 |
| y4 | 446.26 | 26.0 |
| y5 | 561.29 | 26.0 |
| y6 | 708.36 | 26.0 |
| y7 | 836.42 | 26.0 |
| y8 | 949.5 | 26.0 |
| y9 | 1096.57 | 26.0 |
2.6 Calibration Curve of Standard
Description: A dilution series was done on the internal standard from 50 fmol to 0.1 fmol. Sensitivity was found to 1 fmol.
Experiment Description: Cells from colon caner line HCT 116 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer. Sample was analyzed concurrently with internal standard
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
