Information for Peptide: WYVDGVEVHNAK from IGHG3_HUMAN
1. Biological Peptide
1.1 Native Sequence
K.WYVDGVEVHNAK.T
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 207 | 218 | 1415.68 | 708.85 | 472.9 | 5.32 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| W | b | y | ||
| Y | b | y | ||
| V | b | y | ||
| D | b | y | ||
| G | b | y | ||
| V | b | y | ||
| E | b | y | ||
| V | b | y | ||
| H | b | y | ||
| N | b | y | ||
| A | b | y | ||
| K | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
WYVDGVEV(C5N1)HNAK (Modification: Stable Isotope on V8 (U-13C5, 15N) )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 1421.7 | 711.86 | 474.91 | 5.32 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| W | b | y | ||
| Y | b | y | ||
| V | b | y | ||
| D | b | y | ||
| G | b | y | ||
| V | b | y | ||
| E | b | y | ||
| V | b | y | ||
| H | b | y | ||
| N | b | y | ||
| A | b | y | ||
| K | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for WYVDGVEVHNAK
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y6 | 697.363 | 28.0 |
| y8 | 853.453 | 28.0 |
| y9 | 968.48 | 28.0 |
2.6 Calibration Curve of Standard
Description: A dilution series was done on the internal standards from 50 fmol to 0.5 fmols, with sensitivity found down to 10 fmol
Experiment Description: Serum sample digest from healthy adult venous blood draw. Sample denatured in Urea, reduced and alkylated (20mM TCEP and 200mM IAA) and digested overnight with trypsin. Sample was diluted in 2% ACN with 0.1% formic acid and a total of 0.1 nanoliters of serum was injected onto column.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
