Information for Peptide: VTLTSEEEAR from LDHA_HUMAN

1. Biological Peptide

1.1 Native Sequence

    K.VTLTSEEEAR.L

1.2 Peptide Properties

Start	Stop	MW	m/z for 2+	m/z for 3+	pI
306	315	1133.56	567.79	378.86	4.25

1.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
V	b		y
T	b		y
L	b		y
T	b		y
S	b		y
E	b		y
E	b		y
E	b		y
A	b		y
R	b		y

2. Synthetic Peptide

2.1 Synthetic (Internal Standard) Peptide Sequence

VTLTSEEEGR (Modification: A9 to G )

2.2 Peptide Properties

MW	m/z for 2+	m/z for 3+	pI
1119.54	560.78	374.19	4.25

2.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
V	b		y
T	b		y
L	b		y
T	b		y
S	b		y
E	b		y
E	b		y
E	b		y
G	b		y
R	b		y

2.4 Peptide Synthesis Report

Click to view/download synthesis report for VTLTSEEEAR

2.5 QqQ MS/MS

Transition Table:

Transition	m/z of Transition	Collision Energy
b3	314.20803	23.0
b4	415.2557	20.0
b5	502.28773	22.0
y3	361.1836	35.0
y4	490.22619	22.0
y5	619.26879	20.0
y6	706.30082	21.0
y7	807.34849	20.0
y8	920.43256	24.0
y9	1021.4802	22.0

2.6 Calibration Curve of Standard

Description: NONE

 

Experiment Description: Cells from HCT116 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.

2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times

2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides