Information for Peptide: VTDALNATR from CH60_HUMAN
1. Biological Peptide
1.1 Native Sequence
R.VTDALNATR.A
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 421 | 429 | 959.5 | 480.76 | 320.84 | 5.81 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| V | b | y | ||
| T | b | y | ||
| D | b | y | ||
| A | b | y | ||
| L | b | y | ||
| N | b | y | ||
| A | b | y | ||
| T | b | y | ||
| R | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
VTDALNGTR (Modification: A7 to G )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 945.49 | 473.75 | 316.17 | 5.81 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| V | b | y | ||
| T | b | y | ||
| D | b | y | ||
| A | b | y | ||
| L | b | y | ||
| N | b | y | ||
| G | b | y | ||
| T | b | y | ||
| R | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for VTDALNATR
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y8 | 847.42741 | 20.0 |
| y7 | 746.37973 | 19.0 |
| y6 | 631.35279 | 22.0 |
| y5 | 560.31568 | 20.0 |
| y4 | 447.23161 | 18.0 |
2.6 Calibration Curve of Standard
Description: NONE
Experiment Description: Cells from line 8226 were lysed in 8M urea/100mM ammonium bicarbonate and then were subjected to in-solution digestion with trypsin, following reduction and alkylation. The digest was then diluted down to the equivalent of 500 cells and injected concurrently with internal standards into a TSQ QqQ Mass spectrometer.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
