Information for Peptide: VLGTEELYGYLK from CSK22_HUMAN
1. Biological Peptide
1.1 Native Sequence
K.VLGTEELYGYLK.K
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 249 | 260 | 1383.73 | 692.87 | 462.25 | 4.53 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| V | b | y | ||
| L | b | y | ||
| G | b | y | ||
| T | b | y | ||
| E | b | y | ||
| E | b | y | ||
| L | b | y | ||
| Y | b | y | ||
| G | b | y | ||
| Y | b | y | ||
| L | b | y | ||
| K | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
VLGTEELYGYL(C6N1)K (Modification: Stable Isotope on L11 (U-13C6, 15N) )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 1390.75 | 696.38 | 464.59 | 4.53 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| V | b | y | ||
| L | b | y | ||
| G | b | y | ||
| T | b | y | ||
| E | b | y | ||
| E | b | y | ||
| L | b | y | ||
| Y | b | y | ||
| G | b | y | ||
| Y | b | y | ||
| L | b | y | ||
| K | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for VLGTEELYGYLK
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y3 | 430.26 | 21.0 |
| y4 | 487.28 | 28.0 |
| y5 | 650.35 | 24.0 |
| y6 | 763.43 | 24.0 |
| y7 | 892.47 | 24.0 |
| y8 | 1021.51 | 18.0 |
| y9 | 1122.56 | 16.0 |
| y10 | 1179.58 | 21.0 |
| y11 | 1292.67 | 26.0 |
2.6 Calibration Curve of Standard
Description: NONE
Experiment Description: Cells from colon caner line HCT 116 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer. Sample was analyzed concurrently with internal standard
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
