Information for Peptide: VLELDSVK from 2AAB_HUMAN
1. Biological Peptide
1.1 Native Sequence
K.VLELDSVK.S
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 207 | 214 | 901.51 | 451.76 | 301.51 | 4.37 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| V | b | y | ||
| L | b | y | ||
| E | b | y | ||
| L | b | y | ||
| D | b | y | ||
| S | b | y | ||
| V | b | y | ||
| K | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
VLELDSV(C65N1)K (Modification: Stable Isotope on V7 (u-13C5, 15N) )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 967.73 | 484.87 | 323.58 | 4.37 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| V | b | y | ||
| L | b | y | ||
| E | b | y | ||
| L | b | y | ||
| D | b | y | ||
| S | b | y | ||
| V | b | y | ||
| K | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for VLELDSVK
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y3 | 339.21 | 21.0 |
| y4 | 454.24 | 19.0 |
| y5 | 567.32 | 16.0 |
| y6 | 696.37 | 14.0 |
| y7 | 809.45 | 17.0 |
2.6 Calibration Curve of Standard
Description: Internal standard was diluted from 50 fmol to 500 amol injection concentrations and was found to be sensitive down to 0.5 fmol
Experiment Description: Cells from colon caner line HCT 116 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
