Information for Peptide: TVDNLLEVSAR from HSPB2_HUMAN
1. Biological Peptide
1.1 Native Sequence
R.TVDNLLEVSAR.H
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 92 | 102 | 1215.65 | 608.83 | 406.22 | 4.37 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| T | b | y | ||
| V | b | y | ||
| D | b | y | ||
| N | b | y | ||
| L | b | y | ||
| L | b | y | ||
| E | b | y | ||
| V | b | y | ||
| S | b | y | ||
| A | b | y | ||
| R | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
(NONE)
2.2 Peptide Properties
(NONE)
2.3 Fragment Ion Table
(NONE)
2.4 Peptide Synthesis Report
(NONE)
2.5 QqQ MS/MS
2.6 Calibration Curve of Standard
Description: NONE
Experiment Description: Cells from Myeloma cell line RPMI-8226 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. In solution digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. the equivilant of 50000 cells was was then analyzed on a Thermo Scientific TSQ mass spectrometer. Peptides and transitions for analysis were selected using a predictive peptide program. The results were screened for those with the highest specificity and the most intense transitions.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
