Information for Peptide: TSDTETEPSK from KI67_HUMAN

1. Biological Peptide

1.1 Native Sequence

    K.TSDTETEPSK.T

1.2 Peptide Properties

Start	Stop	MW	m/z for 2+	m/z for 3+	pI
858	867	1093.48	547.75	365.5	4.14

1.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
T	b		y
S	b		y
D	b		y
T	b		y
E	b		y
T	b		y
E	b		y
P	b		y
S	b		y
K	b		y

2. Synthetic Peptide

2.1 Synthetic (Internal Standard) Peptide Sequence

TSDTETEP(C5N1)SK (Modification: Stable Isotope on P8 )

2.2 Peptide Properties

MW	m/z for 2+	m/z for 3+	pI
1099.49	550.75	367.5	4.14

2.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
T	b		y
S	b		y
D	b		y
T	b		y
E	b		y
T	b		y
E	b		y
P	b		y
S	b		y
K	b		y

2.4 Peptide Synthesis Report

Click to view/download synthesis report for TSDTETEPSK

2.5 QqQ MS/MS

Transition Table:

Transition	m/z of Transition	Collision Energy
y3	337.19	20.0
y4	466.24	20.0
y5	567.29	20.0
y6	696.33	20.0
y9	999.44	20.0

2.6 Calibration Curve of Standard

Description: NONE

 

Experiment Description: Cells from Breast cancer MCF7 cell line were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer. Internal standard has been synthesized and is awaiting analysis with sample

2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times

2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides