Information for Peptide: TPLTATLSK from IGHA1_HUMAN
1. Biological Peptide
1.1 Native Sequence
K.TPLTATLSK.S
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 213 | 221 | 930.54 | 466.28 | 311.19 | 8.41 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| T | b | y | ||
| P | b | y | ||
| L | b | y | ||
| T | b | y | ||
| A | b | y | ||
| T | b | y | ||
| L | b | y | ||
| S | b | y | ||
| K | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
TPLTATL(C5N1)SK (Modification: Stable Isotope on L7 (U-13C6, 15N) )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 936.55 | 469.28 | 313.19 | 8.41 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| T | b | y | ||
| P | b | y | ||
| L | b | y | ||
| T | b | y | ||
| A | b | y | ||
| T | b | y | ||
| L | b | y | ||
| S | b | y | ||
| K | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for TPLTATLSK
2.5 QqQ MS/MS
2.6 Calibration Curve of Standard
Description: Internal standard TPLTATLSK was diluted from 50 fmol to 500 amol injection concentrations and was found to be sensitive down to 1 fmol
Experiment Description: Serum sample digest from healthy adult venous blood draw. Sample denatured in Urea, reduced and alkylated (20mM TCEP and 200mM IAA) and digested overnight with trypsin. Sample was diluted in 2% ACN with 0.1% formic acid and a total of 0.1 nanoliters of serum was injected onto column.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
