Information for Peptide: TIVTTLQDSIR from TSP1_HUMAN
1. Biological Peptide
1.1 Native Sequence
R.TIVTTLQDSIR.K
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 289 | 299 | 1245.69 | 623.85 | 416.24 | 5.5 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| T | b | y | ||
| I | b | y | ||
| V | b | y | ||
| T | b | y | ||
| T | b | y | ||
| L | b | y | ||
| Q | b | y | ||
| D | b | y | ||
| S | b | y | ||
| I | b | y | ||
| R | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
TIVTTLQESIR (Modification: D8 to E )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 1259.71 | 630.86 | 420.91 | 5.66 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| T | b | y | ||
| I | b | y | ||
| V | b | y | ||
| T | b | y | ||
| T | b | y | ||
| L | b | y | ||
| Q | b | y | ||
| E | b | y | ||
| S | b | y | ||
| I | b | y | ||
| R | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for TIVTTLQDSIR
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y9 | 1046.58464 | 24.5 |
| y8 | 947.51623 | 24.5 |
| y7 | 846.46855 | 24.5 |
| y6 | 745.42087 | 24.5 |
2.6 Calibration Curve of Standard
Description: NONE
Experiment Description: Control urine was processed via ultrafiltration according to Moffitt Proteomics protocol and subjected to in-solution digestion with trypsin, following thermal denaturation, reduction, and alkylation.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
